E-Selectin/CD62E Antibody (CL2/6)

Product Discontinued

E-Selectin/CD62E Antibody (CL2/6) Summary

• Immunogen: Made to Human CD62E
• Specificity: NB100-64915 recognizes human CD62E, a member of the selectin family which is expressed on endothelial cells at sites of inflammation. Clone CL2/6 has been shown to inhibit the binding of human neutrophils to stimulated HUVECS. Removal of sodium azide is recommended prior to use in functional assays.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELE
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 0.5 ug/ml-2 ug/ml
• Control:

  E-Selectin/CD62E Overexpression Lysate

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for E-Selectin/CD62E Antibody (CL2/6)

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signaling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. The main ligands recognized by CD62E are oligosaccharides related to sialyl lewis x.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SELE
• Entrez:
  • 6401(Human)
• Uniprot:
  • P16581()