E-Selectin/CD62E Antibody (BBIG-E5) [Fluorescein]

Human umbilical cord endothelial cells cultured for 4 hours in the presence of 10 ng/mL of rhTNF-a were stained with anti-human CD62E-Fluorescein (R&D Systems, Cat. # BBA21, filled histogram) or with isotype control (R&D Systems, Cat. # IC002F, open histogram).

• Reactivity: Hu
• Applications: Flow
• Clone: BBIG-E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein
• Concentration: LYOPH

E-Selectin/CD62E Antibody (BBIG-E5) [Fluorescein] Summary

• Immunogen: Activated HUVEC human umbilical vein endothelial cells
• Specificity: Detects human E‑Selectin/CD62E
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELE
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide
• Concentration: LYOPH

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin/CD62E Antibody (BBIG-E5) [Fluorescein]

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E) is a 115 kDa type-I membrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines such as IL-1 beta and TNF-alpha or endotoxin (1 - 4). Expression is transitory reaching a maximum within 6 hours of stimulation and then declining with the generation of a soluble form of E-Selectin (1 - 5). Expression of E-Selectin on cell surfaces facilitates the rolling attachment of leukocytes to the endothelium which is an important step in the extravasation of leukocytes at sites of inflammation (1 - 6). E-Selectin is thought to play a prominent role in inflammatory precesses of the skin (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin/CD62E Antibody (BBA21)(11)

Publications using BBA21

• D Eckert, F Rapp, AT Tsedeke, J Molendowsk, R Lehn, M Langhans, C Fournier, F Rödel, S Hehlgans ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells Cells, 2021-12-27;11(1):. 2021-12-27 [PMID: 35011634] (Flow Cytometry, Human)
• Bone Morphogenetic Protein 9 Enhances Lipopolysaccharide-Induced Leukocyte Recruitment to the Vascular Endothelium J Immunol, 2016-09-19;0(0):. 2016-09-19 [PMID: 27647829] (Flow Cytometry, Human)
• Wilhelmsen K, Khakpour S, Tran A, Sheehan K, Schumacher M, Xu F, Hellman J The endocannabinoid/endovanilloid N-arachidonoyl dopamine (NADA) and synthetic cannabinoid WIN55,212-2 abate the inflammatory activation of human endothelial cells. J Biol Chem, 2014-03-18;289(19):13079-100. 2014-03-18 [PMID: 24644287] (Flow Cytometry, Human)
• Stephan D, Sbai O, Wen J, Couraud P, Putterman C, Khrestchatisky M, Desplat-Jego S TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier. J Neuroinflammation, 2013-01-15;10(0):9. 2013-01-15 [PMID: 23320797] (Flow Cytometry, Human)
• Qiang Shi, Gerald Schatten, Vida Hodara, Calvin Simerly, John L. VandeBerg Endothelial reconstitution by CD34+ progenitors derived from baboon embryonic stem cells Journal of Cellular and Molecular Medicine 2/1/2013 [PMID: 23301772]
• Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. J Biol Chem, 2012-06-15;287(32):26478-94. 2012-06-15 [PMID: 22707717] (Flow Cytometry, Human)
• Huang RB, Eniola-Adefeso O Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells. PLoS ONE, 2012-02-24;7(2):e31874. 2012-02-24 [PMID: 22384091] (ICC, Human)
• Qiang Shi, Laura A. Cox, Vida Hodara, Xing Li Wang, John L. VandeBerg Repertoire of endothelial progenitor cells mobilized by femoral artery ligation: a nonhuman primate study Journal of Cellular and Molecular Medicine 9/1/2012 [PMID: 22128816]
• Ying I. Wang, John Schulze, Nadine Raymond, Tyler Tomita, Kayan Tam, Scott I. Simon et al. Endothelial inflammation correlates with subject triglycerides and waist size after a high-fat meal American Journal of Physiology-Heart and Circulatory Physiology 3/1/2011 [PMID: 21169396]
• Gutierrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montano LF, Lopez-Marure R Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells. Atherosclerosis, 2006-03-30;190(1):90-9. 2006-03-30 [PMID: 16574124] (Flow Cytometry, Human)
• Satchell SC, Anderson KL, Mathieson PW Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties. J. Am. Soc. Nephrol., 2004-03-01;15(3):566-74. 2004-03-01 [PMID: 14978158] (ICC, Human)

Bioinformatics

• Gene Symbol: SELE