DYRK2 Antibody

Western Blot: DYRK2 Antibody [NB110-94452] - Detection of DYRK2 in 293 whole cell lysates using NB110-94452.

Immunocytochemistry/Immunofluorescence: DYRK2 Antibody [NB110-94452] - DYRK2 antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Discontinued

DYRK2 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of human DYRK2 (within residues 400-500). [Swiss-Prot# Q92630]
• Localization: Cytoplasm. Nucleus. Note=Translocates into the nucleus following DNA damage.
• Predicted Species: Zebrafish (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DYRK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:50
  • Western Blot 2 ug/ml
• Application Notes: This DYRK2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~60 kDa. In ICC/IF cytoplasmic staining was observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  293 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DYRK2 Antibody

• dual specificity tyrosine-phosphorylation-regulated kinase 2
• dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
• DYRK2
• EC 2.7.12
• EC 2.7.12.1
• FLJ21217
• FLJ21365

Background

DYRK2, or dual specificity tyrosine-phosphorylation-regulated kinase 2, has a role in the regulation of cellular growth and/or development. In response to DNA damage, DYRK2 regulates p53 phosphorylation on Ser-46 to induce apoptosis. DYRK2 has been proposed to inactivate GYS1 by phosphorylation at Ser-641, and potentially a second phosphorylation site, thus regulating glycogen synthesis. DYRK2 also phosphorylates EIF2B5 at Ser-544, enabling its subsequent phosphorylation and inhibition by GSK3, and may play a more general role in the priming of GSK3 substrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DYRK2
• Entrez:
  • 8445(Human)
  • 69181(Mouse)
  • 314862(Rat)
• Uniprot:
  • Q92630()