DYRK2 Antibody (599542) [Alexa Fluor® 350]

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: 599542
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

DYRK2 Antibody (599542) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human DYRK2
  aa 1-107
  Accession # NP_003574
• Specificity: Detects human and rat DYRK2 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DYRK2 Antibody (599542) [Alexa Fluor® 350]

• dual specificity tyrosine-phosphorylation-regulated kinase 2
• dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
• DYRK2
• EC 2.7.12
• EC 2.7.12.1
• FLJ21217
• FLJ21365

Background

DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a 58‑62 kDa member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. It is expressed in testis and shows dual substrate specificity; autophosphorylation on Tyr382 to self‑activate, and a Ser/Thr phosphorylation of target molecules. Substrates include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 initiates cell apoptosis. Human DYRK2 is 601 amino acids (aa) in length. It contains one kinase catalytic domain (aa 222‑535). One isoform variant shows an alternate start site at Met74. This shorter isoform was used for immunization. Thus, over aa 1‑107 (or aa 74‑180 of the long form), human DYRK2 shares 93% aa identity with mouse DYRK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.