Dynamin Antibody

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Western Blot: Dynamin Antibody [NB300-565] - Analysis of rat brain homogenate.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Dynamin Antibody Summary

Immunogen
Synthetic Peptide corresponding to resdiues C K(633) E K A S E T E E N G S D S F(647) of rat Dyn1.
Localization
Microtubule associated.
Specificity
Dynamin 1 - Synapse
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNM1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry 1:10 - 1:500
Application Notes
WB: Detects an approx. 100 kDa protein representing Dynamin 1 from rat whole brain extract.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dynamin Antibody

  • DNM
  • DNM1
  • dynamin 1
  • Dynamin
  • dynamin-1
  • EC 3.6.5.5

Background

The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin I is expressed exclusively in neurons, dynamin II is ubiquitously expressed, and dynamin III is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DNM1
Entrez
Uniprot