Dynamin Antibody

Western Blot: Dynamin Antibody [NB300-565] - Analysis of rat brain homogenate.

Product Discontinued

Dynamin Antibody Summary

• Immunogen: Synthetic Peptide corresponding to resdiues C K(633) E K A S E T E E N G S D S F(647) of rat Dyn1.
• Localization: Microtubule associated.
• Specificity: Dynamin 1 - Synapse
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 100 kDa protein representing Dynamin 1 from rat whole brain extract.
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Dynamin Antibody

• DNM
• DNM1
• dynamin 1
• Dynamin
• dynamin-1
• EC 3.6.5.5

Background

The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin I is expressed exclusively in neurons, dynamin II is ubiquitously expressed, and dynamin III is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNM1
• Entrez:
  • 1759(Human)
  • 140694(Rat)
• Uniprot:
  • P21575(Rat)