Dynamin [p Ser778] Antibody - Azide Free

Western Blot: Dynamin [p Ser778] Antibody [NB300-210] - Analysis of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Ch
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: Azide Free

Dynamin [p Ser778] Antibody - Azide Free Summary

• Description: Novus Biologicals Sheep Dynamin [p Ser778] Antibody - Azide Free (NB300-210) is a polyclonal antibody validated for use in WB. Anti-Dynamin Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 conjugated to KLH. Accession # P21575
• Modification: p Ser778
• Localization: Cytoplasmic
• Specificity: Specific for endogenous levels of the ~95 kDa dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Canine (100%), Chicken (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DNM1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: This antibody is useful in Western blot where a band is seen at ~ 95kD, representing Dynamin. Immunohistochemistry and Immunofluorescence have not been tested.
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26658127)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Dynamin [p Ser778] Antibody - Azide Free

• DNM
• DNM1
• dynamin 1
• Dynamin
• dynamin-1
• EC 3.6.5.5

Background

Dynamins are a family of GTPases transcribed from at least three separate genes. They contain several conserved regions; an amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a region located in front of the proline-rich domain (PRD). This PRD region is thought to mediate interactions between dynamin and other cellular proteins. There are also at least three isoforms of this protein. Dynamin 2 is ubiquitous. Dynamin 3 is expressed in the brain, testis, heart and lung. Dynamin 1 is exclusively neuronal and is phosphorylated by PKC and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is believed that this dephosphorylation provides a trigger for the cellular process of clatherin-mediated and fluid-phase endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dynamin Antibody (NB300-210)(3)

Publications using NB300-210

• Du JJ, Yan L, Zhang W et al. Clathrin-independent but dynamin-dependent mechanisms mediate Ca2+-triggered endocytosis of the glutamate GluK2 receptor upon excitotoxicity Journal of Integrative Neuroscience 2020-09-30 [PMID: 33070524]
• Patel A, Yamashita N, Ascano M et al. RCAN1 links impaired neurotrophin trafficking to aberrant development of the sympathetic nervous system in Down syndrome. Nat Commun. 2015-12-15 [PMID: 26658127] (WB, Mouse)
  
  Details:
  phospho-Dynamin [Ser778] antibody was used for WB analysis of lysates from salivary glands of mouse pups P1 (mouse model of Down syndrome) and NGF stimulated sympathetic neurons of P0.5 Sprague–Dawley rats. The lysates were prepared in Laemmli buffer (62.5?mM Tris-HCl, 2% SDS, 10% Glycerol, 5% 2-mercaptoethanol, 0.01% bromophenol blue) and the antibody was employed at 1:5000 dilution.
• Tan, TC et al. Cdk5 is Essential for Synaptic Vesicle Endocytosis. Nat. Cell Bio., 5(8): 701-710. 2003-01-01 [PMID: 12855954]

Bioinformatics

• Gene Symbol: DNM1
• Entrez:
  • 1759(Human)
  • 13429(Mouse)
  • 140694(Rat)
• Uniprot:
  • P21575(Rat)