Dynamin [p Ser774] Antibody

Images

 
Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of ...read more

Product Details

Summary
Reactivity Rt, Hu, Mu, Bv, Ca, Ch, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated

Order Details

Dynamin [p Ser774] Antibody Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer774 of rat dynamin.
Modification
p Ser774
Localization
Cytoplasmic
Specificity
Specific for the ~95k dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.
Predicted Species
Human (100%), Mouse (100%), Canine (100%), Chicken (100%), Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
DNM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence 1:10 - 1:500
Application Notes
NB300-209 can be used in Western blot where a band is seen at ~ 97 kD, representing Dynamin
Publications
Read Publication using NB300-209.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for Dynamin [p Ser774] Antibody

  • DNM
  • DNM1
  • dynamin 1
  • Dynamin
  • dynamin-1
  • EC 3.6.5.5

Background

Dynamins are a family of GTPases transcribed from at least three separate genes. They contain several conserved regions; an amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a region located in front of the proline-rich domain (PRD). This PRD region is thought to mediate interactions between dynamin and other cellular proteins. There are also at least three isoforms of this protein. Dynamin 2 is ubiquitous. Dynamin 3 is expressed in the brain, testis, heart and lung. Dynamin 1 is exclusively neuronal and is phosphorylated by PKC and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is believed that this dephosphorylation provides a trigger for the cellular process of clatherin-mediated and fluid-phase endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, IF

Publications for Dynamin Antibody (NB300-209)(1)


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Bioinformatics

Gene Symbol DNM1
Entrez
Uniprot