Dynamin 2 Antibody (JM61-38)

Western Blot: Dynamin 2 Antibody (JM61-38) [NBP2-66813] - Analysis of Dynamin 2 on different lysates using anti-Dynamin 2 antibody at 1/1,000 dilution.Positive control:Lane1: PC-12Lane2: Mouse thymusLane3: Hela

Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody (JM61-38) [NBP2-66813] - Staining Dynamin 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Dynamin 2 Antibody (JM61-38) [NBP2-66813] - Analysis of paraffin-embedded mouse testes tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Dynamin 2 Antibody (JM61-38) [NBP2-66813] - Analysis of Hela cells with Dynamin 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody (JM61-38) [NBP2-66813] - Staining Dynamin 2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Dynamin 2 Antibody (JM61-38) [NBP2-66813] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Dynamin 2 Antibody (JM61-38) [NBP2-66813] - Analysis of paraffin-embedded human kidney tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Dynamin 2 Antibody (JM61-38) [NBP2-66813] - Analysis of paraffin-embedded mouse colon tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Dynamin 2 Antibody (JM61-38) [NBP2-66813] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.

Product Discontinued

Dynamin 2 Antibody (JM61-38) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Dynamin 2 aa 748-797 / 870. (SwissProt: P50570 Human; SwissProt: P39054 Mouse; SwissProt: P39052 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DNM2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Dynamin 2 Antibody (JM61-38)

• CMTDI1CMT2M
• CMTDIBdynamin II
• DI-CMTB
• DNM2
• DYN2
• DYN2EC 3.6.5.5
• Dynamin 2
• dynamin-2
• DYNII

Background

The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNM2