Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [H00010540-B01P] - Analysis of DCTN2 expression in human spleen.

Immunocytochemistry/Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [H00010540-B01P] - Analysis of purified antibody to DCTN2 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [H00010540-B01P] - Analysis of DCTN2 expression in transfected 293T cell line by DCTN2 polyclonal antibody. Lane 1: DCTN2 transfected lysate(44.11 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Dynactin Subunit 2/DCTN2/DCTN-50 Antibody Summary

• Immunogen: DCTN2 (AAH00718.1, 1 a.a. - 401 a.a.) full-length human protein. MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK
• Specificity: DCTN2 - dynactin 2 (p50),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DCTN2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It is also useful for immunofluoresence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

• DCTN2
• DCTN-50
• DCTN5050 kD dynein-associated polypeptide
• dynactin 2 (p50)
• dynactin complex 50 kD subunit
• Dynactin complex 50 kDa subunit
• Dynactin Subunit 2
• dynactin subunit 2,50 kDa dynein-associated polypeptide
• Dynamitin
• p50 dynamitin
• RBP50

Background

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody (H00010540-B01P)(1)

Publication using H00010540-B01P

• Chatin B, Colombier P, Gamblin AL et al. Dynamitin Impacts Cell Surface Expression of Voltage-Gated Sodium Channel Nav1.5. Biochem J. 2014-08-04 [PMID: 25088759]

Bioinformatics

• Gene Symbol: DCTN2
• Entrez:
  • 10540(Human)
• Uniprot:
  • AAH00718.1(Human)