DUSP8 Antibody (HL2338)

MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DUSP8 stained by DUSP8 antibody [HL2338] diluted at 1:500.Blue: Fluoroshield with DAPI.

Human tissue extract (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (NBP3-48677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (NBP3-48677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (NBP3-48677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clone: HL2338
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

DUSP8 Antibody (HL2338) Summary

• Description: Novus Biologicals Rabbit DUSP8 Antibody (HL2338) (NBP3-48677) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human DUSP8
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DUSP8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for DUSP8 Antibody (HL2338)

• C11orf81
• chromosome 11 open reading frame 81
• dual specificity phosphatase 8
• Dual specificity protein phosphatase hVH-5
• EC 3.1.3.16
• EC 3.1.3.48
• FLJ42476
• FLJ42958
• HVH8
• serine/threonine specific protein phosphatase
• vaccinia virus homolog
• VH5

Background

DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8 inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DUSP8