DUSP14 Antibody

Immunohistochemistry-Paraffin: DUSP14 Antibody [NB100-887] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DUSP14 Antibody Summary

• Description: Novus Biologicals Goat DUSP14 Antibody (NB100-887) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-DUSP14 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CEKESRHLMPYWGI corresponding to C-Terminus according to NP_008957.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DUSP14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.5 ug/ml
  • Immunohistochemistry-Paraffin 3.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 ug/ml
• Application Notes: WB: No signal obtained yet ,but low background observed in human liver extracts at up to 1 ug/ml. IHC: Paraffin embedded Human Testis.
• Control:

  DUSP14 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DUSP14 Antibody

• dual specificity phosphatase 14
• EC 3.1.3.16
• EC 3.1.3.48
• MAP kinase phosphatase 6
• Mitogen-activated protein kinase phosphatase 6
• MKP-1 like protein tyrosine phosphatase
• MKP-1-like protein tyrosine phosphatase
• MKP-6
• MKP6MKP-Ldual specificity protein phosphatase 14

Background

In addition to antigen recognition by the T-cell receptor, T-cell activation requires a second signal from a costimulatory receptor, such as CD28 which interacts with B7-1 and B7-2 ligands on antigen-presenting cells. CD28 co stimulation induces transcription of interleukin-2 and stabilizes newly synthesized IL2 through the activation of mitogen-activated protein kinases (MAPKs), such as ERK and JNK, and the subsequent creation of AP1 transcription factor. DUSP14 is a negative regulator of CD28 signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUSP14 Antibody (NB100-887)(1)

Publication using NB100-887

• Marti F, Krause A, Post NH et al. Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol 2001-01-01 [PMID: 11123293]

Bioinformatics

• Gene Symbol: DUSP14
• Entrez:
  • 11072(Human)
  • 56405(Mouse)
• Uniprot:
  • NP_008957.(Human)