DUSP14 Antibody (4B5-E6) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: DUSP14 Antibody (4B5-E6) [H00011072-M02] - Analysis of monoclonal antibody to DUSP14 on HeLa cell . Antibody concentration 10 ug/ml.

ELISA: DUSP14 Antibody (4B5-E6) [H00011072-M02] - Detection limit for recombinant GST tagged DUSP14 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4B5-E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DUSP14 Antibody (4B5-E6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DUSP14 Antibody (4B5-E6) - Azide and BSA Free (H00011072-M02) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-DUSP14 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: DUSP14 (AAH00370, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSRGHSTLPRTLMAPRMISEGDIGGIAQITSSLFLGRGSVASNRHLLQARGITCIVNATIEIPNFNWPQFEYVKVPLADMPHAPIGLYFDTVADKIHSVSRKHGATLVHCAAGVSRSATLCIAYLMKFHNVCLLEAYNWVKARRPVIRPNVGFWRQLIDYERQLFGKSTVKMVQTPYGIVPDVYEKESRHLMPYWGI
• Specificity: DUSP14 - dual specificity phosphatase 14
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DUSP14
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DUSP14 Antibody (4B5-E6) - Azide and BSA Free

• dual specificity phosphatase 14
• EC 3.1.3.16
• EC 3.1.3.48
• MAP kinase phosphatase 6
• Mitogen-activated protein kinase phosphatase 6
• MKP-1 like protein tyrosine phosphatase
• MKP-1-like protein tyrosine phosphatase
• MKP-6
• MKP6MKP-Ldual specificity protein phosphatase 14

Background

In addition to antigen recognition by the T-cell receptor, T-cell activation requires a second signal from a costimulatory receptor, such as CD28 (MIM 186760), which interacts with B7-1 (CD80; MIM 112203) and B7-2 (CD86; MIM 601020) ligands on antigen-presenting cells. CD28 costimulation induces transcription of interleukin-2 (IL2; MIM 147680) and stabilizes newly synthesized IL2 through the activation of mitogen-activated protein kinases (MAPKs), such as ERK (e.g., MAP2K4; MIM 601335) and JNK (see MIM 601158), and the subsequent creation of AP1 transcription factor (see MIM 165160). DUSP14 is a negative regulator of CD28 signaling.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUSP14 Antibody (H00011072-M02)(1)

Publication using H00011072-M02

• Yang Chia-Yu, Li Ju-Pi, Chiu Li-Li et al. Dual-specificity phosphatase 14 (DUSP14/MKP6) negatively regulates TCR signaling by inhibiting TAB1 activation. J Immunol. 2014-02-15 [PMID: 24403530]

Bioinformatics

• Gene Symbol: DUSP14
• Entrez:
  • 11072(Human)
• OMIM:
  • 606618(Human)
• Uniprot:
  • AAH00370(Human)