DSPG3/EPYC Antibody (329707) [Unconjugated]

DSPG3 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human DSPG3 Monoclonal Antibody (Catalog # MAB3110) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 329707
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DSPG3/EPYC Antibody (329707) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DSPG3
  Ala20-Val322
  Accession # Q99645
• Specificity: Detects human DSPG3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse DSPG3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPYC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DSPG3/EPYC Antibody (329707) [Unconjugated]

• Dermatan sulfate proteoglycan 3dermatan sulphate proteoglycan 3
• DSPG3
• DSPG3proteoglycan-lb
• Epiphycan
• EPYC
• PGLB
• PG-Lb
• Proteoglycan-Lb
• SLRR3B
• SLRR3Bepiphycan proteoglycan
• Small chondroitin/dermatan sulfate proteoglycan

Background

Human DSPG3 (also PG-Lb and epiphycan) is a 95‑100 kDa class III subfamily member of the SLRP family of molecules. It is synthesized as a 322 aa precursor that contains a 19 aa signal sequence and a 303 aa mature region. The mature molecule contains two intrachain disulfide bonds that flank six leucine-rich repeats lying between aa 135-301. There is a potential for both O- and N-linked glycosylation. DSPG3 is found principally in cartilage. Mature human DSPG3 is 85%, 96%, 95% and 84% aa identical to rat, dog, cow and mouse DSPG3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPYC
• Uniprot:
  • Q99645()