DSCR4 Overexpression Lysate (Native)

Images

 
Western Blot: DSCR4 Overexpression Lysate (Adult Normal) [NBL1-10025] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DSCR4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DSCR4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210247 and based on accession number NM_005867. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Down syndrome critical region gene 4 (DSCR4), mRNA.
Gene
DSCR4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DSCR4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DSCR4 Overexpression Lysate (Native)

  • DCRBDown syndrome critical region protein B, 10DSCRBDown syndrome critical region protein 4
  • Down syndrome critical region gene 4

Background

The region of chromosome 21 between genes CBR and ERG (CBR-ERG region), which spans 2.5 Mb on 21q22.2, has been defined by analysis of patients with partial trisomy 21. It contributes significantly to the pathogenesis of many characteristics of Down syndrome, including morphological features, hypotonia, and mental retardation. This gene is found in this region and multiple transcripts may exist. It is mainly expressed in the placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-84294
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-46852
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-86687
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-82648
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84975
Species: Hu
Applications: WB, IHC, IHC-P
AF6116
Species: Hu
Applications: WB, ICC
NB100-74575
Species: Hu
Applications: WB, PEP-ELISA
NBP1-76995
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-60052
Species: Hu
Applications: WB
NBP2-02450
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83091
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-84296
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-88527
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00253738-M05
Species: Hu, Mu
Applications: WB, ELISA, IHC, IHC-P
NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB110-58870
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF
NBP1-90187
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-10025
Species: Hu
Applications: WB

Publications for DSCR4 Lysate (NBL1-10025) (0)

There are no publications for DSCR4 Lysate (NBL1-10025).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DSCR4 Lysate (NBL1-10025) (0)

There are no reviews for DSCR4 Lysate (NBL1-10025). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DSCR4 Lysate (NBL1-10025) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DSCR4 Products

Bioinformatics Tool for DSCR4 Lysate (NBL1-10025)

Discover related pathways, diseases and genes to DSCR4 Lysate (NBL1-10025). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DSCR4 Lysate (NBL1-10025)

Discover more about diseases related to DSCR4 Lysate (NBL1-10025).
 

Pathways for DSCR4 Lysate (NBL1-10025)

View related products by pathway.

PTMs for DSCR4 Lysate (NBL1-10025)

Learn more about PTMs related to DSCR4 Lysate (NBL1-10025).

Blogs on DSCR4

There are no specific blogs for DSCR4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

DSCR4 Antibody
NBP1-84295

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DSCR4 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DSCR4