DRAM Antibody

Product Discontinued

DRAM Antibody Summary

• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human DRAM protein.
• Specificity: This is directed against human DRAM protein. This reacts with DRAM from human, mouse and rat sources.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DRAM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:40000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 0.5-2 ug/ml
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Expect a band at approx 33 kDa in size corresponding - DRAM by western blotting in the appropriate cell lysate or extract. K562 cell lysate can be used as positive control.

Reactivity Notes

This antibody is reactive to Human, Mouse, and Rat.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DRAM Antibody

• Damage-regulated autophagy modulator
• DNA damage-regulated autophagy modulator protein 1
• DNA-damage regulated autophagy modulator 1
• DRAMFLJ11259

Background

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DRAM1
• Entrez:
  • 55332(Human)
  • 71712(Mouse)
  • 679937(Rat)
• Uniprot:
  • Q8N682(Human)