DPEP1 Overexpression Lysate (Native)

Images

 
Western Blot: DPEP1 Overexpression Lysate (Adult Normal) [NBL1-09988] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DPEP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DPEP1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201964 and based on accession number NM_004413. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens dipeptidase 1 (renal) (DPEP1), transcript variant 1, mRNA.
Gene
DPEP1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
45.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DPEP1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DPEP1 Overexpression Lysate (Native)

  • dehydropeptidase-I
  • dipeptidase 1 (renal)
  • dipeptidase 1
  • EC 3.4.13.19
  • hRDP
  • MDPMBD1
  • RDPMicrosomal dipeptidase
  • Renal dipeptidase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NB100-56326
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-81657
Species: Hu, Mu
Applications: WB, IHC-P, IP
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
NBP1-86304
Species: Hu
Applications: IHC, IHC-P
NB100-56722
Species: Hu, Ca
Applications: WB, B/N, Flow, Func, ICC/IF, IHC-P, IP, Flow-CS
NB100-56519
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, IHC-FrFl
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NB100-56152
Species: Hu
Applications: WB, IHC, IHC-P, IP
H00008767-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA
AF6567
Species: Hu, Mu
Applications: WB, IHC
NB100-56534
Species: Hu
Applications: WB, IHC-P, ICC
NBP2-24393
Species: Hu
Applications: WB
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-12553
Species: Hu

Publications for DPEP1 Lysate (NBL1-09988) (0)

There are no publications for DPEP1 Lysate (NBL1-09988).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DPEP1 Lysate (NBL1-09988) (0)

There are no reviews for DPEP1 Lysate (NBL1-09988). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DPEP1 Lysate (NBL1-09988) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DPEP1 Products

Bioinformatics Tool for DPEP1 Lysate (NBL1-09988)

Discover related pathways, diseases and genes to DPEP1 Lysate (NBL1-09988). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DPEP1 Lysate (NBL1-09988)

Discover more about diseases related to DPEP1 Lysate (NBL1-09988).
 

Pathways for DPEP1 Lysate (NBL1-09988)

View related products by pathway.

PTMs for DPEP1 Lysate (NBL1-09988)

Learn more about PTMs related to DPEP1 Lysate (NBL1-09988).

Blogs on DPEP1

There are no specific blogs for DPEP1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

DPEP1 Antibody
NBP1-86087

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DPEP1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DPEP1