DP1 Overexpression Lysate (Native)

Images

 
Western Blot: DP1 Overexpression Lysate (Adult Normal) [NBL1-16835] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DP1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203986 and based on accession number NM_007111. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens transcription factor Dp-1 (TFDP1), transcript variant 1, mRNA.
Gene
TFDP1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
44.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DP1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DP1 Overexpression Lysate (Native)

  • DP1E2F dimerization partner 1
  • DRTF1Dp-1
  • DRTF1-polypeptide 1
  • E2F-related transcription factor
  • transcription factor Dp-1

Background

This gene encodes a member of a family of transcription factors that heterodimerize with E2F proteins to enhance their DNA-binding activity and promote transcription from E2F target genes. The encoded protein functions as part of this complex to control the transcriptional activity of numerous genes involved in cell cycle progression from G1 to S phase. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1, 15, and X. Transcript Variant: This variant (1) represents the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for DP1 Lysate (NBL1-16835) (0)

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Bioinformatics

Gene Symbol TFDP1