Doublecortin Overexpression Lysate (Native)

Images

 
Western Blot: Doublecortin Overexpression Lysate (Adult Normal) [NBL1-09760] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Doublecortin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Doublecortin Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221891 and based on accession number NM_178152. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens doublecortin (DCX), transcript variant 2, mRNA.
Gene
DCX

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Doublecortin

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Doublecortin Overexpression Lysate (Native)

  • doublecortin
  • Doublin
  • FLJ51296
  • Lissencephalin-X
  • Lis-X
  • X-linked (doublecortin)

Background

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) contains a distinct 5' UTR, lacks an in-frame portion of the 5' coding region, and uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has a shorter N-terminus and includes an extra segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Doublecortin Lysate (NBL1-09760) (0)

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Video Protocols

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Bioinformatics

Gene Symbol DCX