Dopamine D5R/DRD5 Antibody (889022) [Phycoerythrin]

HEK293 human embryonic kidney cell line transfected with (A) human Dopamine D5 R/DRD5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Dopamine D5 R/DRD5 PE-conjugated Monoclonal Antibody (Catalog # FAB82861P). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

Dopamine D5R/DRD5 Antibody (889022) [Phycoerythrin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
  Met1-His477
  Accession # P21918
• Specificity: Detects HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DRD5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dopamine D5R/DRD5 Antibody (889022) [Phycoerythrin]

• D(1B) dopamine receptor
• D(5) dopamine receptor
• D1beta dopamine receptor
• DBDR
• Dopamine D5 R
• Dopamine D5 receptor
• Dopamine D5R
• dopamine receptor D1B
• dopamine receptor D5
• DRD1B
• DRD1BDBDR
• DRD1L2
• DRD1L2MGC10601
• DRD5

Background

DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of protein kinase C (PKC) (6).

1. Sunahra, R. et al. (1991) Nature. 350:614.
2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
3. Squassina, A. et al.  (2008) Neurosci Lett. Feb 13.
4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.
5. Wei, J. et al.  (2012) Addict Behav. 37:622.
6. Thompson, D. et al. (2011) Traffic. 12:644.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DRD5
• Uniprot:
  • P21918()