DOCK2 Antibody

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human DOCK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DOCK2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DOCK2 Antibody Summary

• Immunogen: E. coli-derived recombinant human DOCK2
  Gly420-Gly642
  Accession # Q92608
• Specificity: Detects human DOCK2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DOCK2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DOCK2 Antibody

• dedicator of cytokinesis 2
• dedicator of cytokinesis protein 2
• DOCK2
• FLJ46592
• KIAA0209dedicator of cyto-kinesis 2

Background

DOCK2 (Dedicator of cytokinesis protein 2) is a member of the DOCK-A family, DOCK180 superfamily of proteins. Its predicted molecular weight is 210 kDa, although it may run approximately at 190 kDa in SDS-PAGE. It is found in hematopoietic cell types, and regulates the activity of the GTPases Rac-1 and -2. Human DOCK2 is 1830 amino acids (aa) in length. It contains one SH3 domain (aa 8‑69), a DHR-1 region that localizes DOCK2 to the plasma membrane (aa 420‑662), and a DHR-2 domain that binds to nucleotide-free GTPases and shows GEF activity (aa 1115‑1621). There are two isoform variants, one that shows a 38 aa substitution for Tyr1405, and a second that shows a start site at Met450 with accompanying deletions of aa 462‑494 and 630‑766, and a 22 aa substitution for aa 934‑1830. Over aa 420‑642, human DOCK2 shares 96% aa identity to mouse DOCK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DOCK2
• Uniprot:
  • Q92608()