| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70583 | |||
HAF109). A specific band was detected for DOCK2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human DOCK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DOCK2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5339-SP | |||
![Western Blot: DOCK2 Antibody (7G2) [NBP2-46468] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOCK2.](http://images.novusbio.com/fullsize/DOCK2-Antibody-7G2-Western-Blot-NBP2-46468-img0008.jpg "Western Blot: DOCK2 Antibody (7G2) [NBP2-46468] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOCK2.")
![Immunohistochemistry: DOCK2 Antibody (7G2) [NBP2-46468] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DOCK2-Antibody-7G2-Immunohistochemistry-NBP2-46468-img0007.jpg)
![Immunohistochemistry: DOCK2 Antibody (7G2) [NBP2-46468] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DOCK2-Antibody-7G2-Immunohistochemistry-NBP2-46468-img0001.jpg)
![Immunohistochemistry: DOCK2 Antibody (7G2) [NBP2-46468] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/DOCK2-Antibody-7G2-Immunohistochemistry-NBP2-46468-img0002.jpg)
![Immunohistochemistry: DOCK2 Antibody (7G2) [NBP2-46468] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DOCK2-Antibody-7G2-Immunohistochemistry-NBP2-46468-img0003.jpg)
![Immunohistochemistry: DOCK2 Antibody (7G2) [NBP2-46468] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DOCK2-Antibody-7G2-Immunohistochemistry-NBP2-46468-img0004.jpg)
![Immunohistochemistry: DOCK2 Antibody (7G2) [NBP2-46468] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DOCK2-Antibody-7G2-Immunohistochemistry-NBP2-46468-img0005.jpg)
![Immunohistochemistry: DOCK2 Antibody (7G2) [NBP2-46468] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DOCK2-Antibody-7G2-Immunohistochemistry-NBP2-46468-img0006.jpg)
![Western Blot: DOCK2 Antibody [NBP2-38303] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10<br/>Lane 2: Human cell line HL-60](http://images.novusbio.com/fullsize/DOCK2-Antibody-Western-Blot-NBP2-38303-img0003.jpg "Western Blot: DOCK2 Antibody [NBP2-38303] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10<br/>Lane 2: Human cell line HL-60")
![Western Blot: DOCK2 Antibody [NBP3-12944] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DOCK2 antibody (NBP3-12944) diluted at 1:1000.](http://images.novusbio.com/fullsize/DOCK2-Antibody-Western-Blot-NBP3-12944-img0001.jpg "Western Blot: DOCK2 Antibody [NBP3-12944] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DOCK2 antibody (NBP3-12944) diluted at 1:1000.")
![SDS-Page: Recombinant Human DOCK2 Protein [H00001794-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001794-P01-1.jpg "SDS-Page: Recombinant Human DOCK2 Protein [H00001794-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunoprecipitation:[NBP3-29705]-Detection of human DOCK2 by western blot of immunoprecipitates. Samples: Whole cell lysate from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK2 antibody used for IP at 6 µg per reaction. DOCK2 was also immunoprecipitated by rabbit anti-DOCK2 antibodies , , and. For blotting immunoprecipitated DOCK2, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29705_rabbit-dock2-pab-23120251252816.jpg "Immunoprecipitation:[NBP3-29705]-Detection of human DOCK2 by western blot of immunoprecipitates. Samples: Whole cell lysate from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK2 antibody used for IP at 6 µg per reaction. DOCK2 was also immunoprecipitated by rabbit anti-DOCK2 antibodies , , and. For blotting immunoprecipitated DOCK2, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: DOCK2 Antibody [NBP1-58870] - THP-1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/DOCK2-Antibody-Western-Blot-NBP1-58870-img0002.jpg "Western Blot: DOCK2 Antibody [NBP1-58870] - THP-1 cell lysate, concentration 0.2-1 ug/ml.")
![Western blot analysis on different lysates with NBP3-32276 at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: THP-1 cell lysate Lane 3: Raji cell lysate Lane 4: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 212 kDa Observed band size: 180 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32276 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32276_-western-blot-639204597147390679.jpg "Western blot analysis on different lysates with NBP3-32276 at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: THP-1 cell lysate Lane 3: Raji cell lysate Lane 4: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 212 kDa Observed band size: 180 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32276 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: DOCK2 Antibody (S04-5H2) [NBP3-19529] - Western blot detection of DOCK2 in Jurkat using NBP3-19529. Predicted band size: 212kDa. Observed band size: 212kDa.](http://images.novusbio.com/fullsize/DOCK2-Antibody-S04-5H2-Western-Blot-NBP3-19529-img0001.jpg "Western Blot: DOCK2 Antibody (S04-5H2) [NBP3-19529] - Western blot detection of DOCK2 in Jurkat using NBP3-19529. Predicted band size: 212kDa. Observed band size: 212kDa.")
![Immunoprecipitation:DOCK2 AntibodyNBP3-29392] -Detection of human DOCK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK2 antibody NBP3-29392 (lot NBP3-29392-1) used for IP at 6 µg per reaction. DOCK2 was also immunoprecipitated by rabbit anti-DOCK2 antibodies . For blotting immunoprecipitated DOCK2, NBP3-29392 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29392_rabbit-dock2-pab-301220241820316.jpg "Immunoprecipitation:DOCK2 AntibodyNBP3-29392] -Detection of human DOCK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK2 antibody NBP3-29392 (lot NBP3-29392-1) used for IP at 6 µg per reaction. DOCK2 was also immunoprecipitated by rabbit anti-DOCK2 antibodies . For blotting immunoprecipitated DOCK2, NBP3-29392 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Proximity Ligation Assay: DOCK2 Antibody (2F4) [H00001794-M01] - Analysis of protein-protein interactions between CD247 and DOCK2. HeLa cells were stained with anti-CD247 rabbit purified polyclonal 1:1200 and anti-DOCK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/DOCK2-Antibody-2F4-Proximity-Ligation-Assay-H00001794-M01-img0001.jpg "Proximity Ligation Assay: DOCK2 Antibody (2F4) [H00001794-M01] - Analysis of protein-protein interactions between CD247 and DOCK2. HeLa cells were stained with anti-CD247 rabbit purified polyclonal 1:1200 and anti-DOCK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
