DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [Biotin]

Images

 
There are currently no images for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Md, Pm, Dr(-)Species Glossary
Applications WB, ICC/IF
Clone
3C5.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [Biotin] Summary

Immunogen
Human DNA Polymerase epsilon purified from HeLa cells.
Localization
Nuclear
Specificity
This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POLE
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This DNA Polymerase epsilon (3C5.1) antibody can be used in Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB, it detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

Mink samples. This antibody does not work on Drosophilia or the baculovirus host cell, and weakly, if at all, on S. cerivisae POLE.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [Biotin]

  • DKFZp434F222
  • DNA polymerase epsilon catalytic subunit A
  • DNA polymerase II subunit A
  • EC 2.7.7
  • EC 2.7.7.7
  • POLE1FLJ21434
  • polymerase (DNA directed), epsilon

Background

DNA polymerase epsilon from HeLa cells differs from yeast pol epsilon in that HeLa pol epsilon contains two subunits of 220 and 55 kDa, whereas yeast pol epsilon has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol epsilon, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol epsilon prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol alpha and pol delta prefer activated DNA and alternating poly(dA-dT) as templates, respectively. Pol epsilon retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol delta. PCNA can also interact with Pol epsilon on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-13785
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-39036
Species: Hu
Applications: IHC, IHC-P
NB100-74611
Species: Hu
Applications: WB, IHC-P, IP
H00010714-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-49672
Species: Hu
Applications: IHC
AF2535
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
NBP2-16391
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-56768
Species: Hu
Applications: ICC/IF
NBP2-13729
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31541
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBL1-15012
Species: Hu
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP1-59904
Species: Hu
Applications: WB, IHC, IHC-P
NB100-230
Species: Hu
Applications: WB, IP, PLA
NB100-61070
Species: Hu
Applications: WB (-), IP
NBP1-83227
Species: Hu, Mu(-)
Applications: WB, IHC, IHC-P
NB100-115B
Species: Hu, Mu, Md, Pm, Dr(-)
Applications: WB, ICC/IF

Publications for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B) (0)

There are no publications for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B) (0)

There are no reviews for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NB100-115UV
DyLight 405 Labeled NB100-115V
DyLight 488 Labeled NB100-115G
DyLight 550 Labeled NB100-115R
DyLight 650 Labeled NB100-115C
DyLight 680 Labeled NB100-115FR
DyLight 755 Labeled NB100-115IR
FITC Labeled NB100-115F
HRP Labeled NB100-115H

Additional DNA Polymerase epsilon catalytic subunit A Products

Bioinformatics Tool for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B)

Discover related pathways, diseases and genes to DNA Polymerase epsilon subunit 3 Antibody (NB100-115B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B)

Discover more about diseases related to DNA Polymerase epsilon subunit 3 Antibody (NB100-115B).
 

Pathways for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B)

View related products by pathway.

PTMs for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B)

Learn more about PTMs related to DNA Polymerase epsilon subunit 3 Antibody (NB100-115B).
 

Research Areas for DNA Polymerase epsilon subunit 3 Antibody (NB100-115B)

Find related products by research area.

Blogs on DNA Polymerase epsilon catalytic subunit A

There are no specific blogs for DNA Polymerase epsilon catalytic subunit A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [Biotin] and receive a gift card or discount.

Bioinformatics

Gene Symbol POLE