DNA polymerase alpha Antibody (3C11)

Western Blot: DNA polymerase alpha Antibody (3C11) [H00005422-M01] - POLA monoclonal antibody (M01), clone 3C11 Analysis of POLA expression in Hela S3 NE.

Sandwich ELISA: DNA polymerase alpha Antibody (3C11) [H00005422-M01] - Detection limit for recombinant GST tagged POLA is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, S-ELISA
• Clone: 3C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DNA polymerase alpha Antibody (3C11) Summary

• Immunogen: POLA (NP_058633, 1363 a.a. ~ 1462 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QFSRTGPLCPACMKATLQPEYSDKSLYTQLCFYRYIFDAECALEKLTTDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSRSGYSEVNLSKLFAGCAVKS
• Specificity: POLA - polymerase (DNA directed), alpha
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNA polymerase alpha Antibody (3C11)

• p180
• DNA polymerase alpha 1 catalytic subunit
• DNA polymerase alpha catalytic subunit p180
• DNA polymerase alpha catalytic subunit
• DNA polymerase alpha p180 subunit
• DNA polymerase alpha
• EC 2.7.7.7
• p180
• POLA
• POLA1
• POLADKFZp686K1672
• polymerase (DNA directed), alpha 1
• polymerase (DNA directed), alpha 1, catalytic subunit
• polymerase (DNA directed), alpha
• polymerase (DNA-directed), alpha (70kD)

Background

DNA polymerase alpha plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLA1
• Entrez:
  • 5422(Human)
• OMIM:
  • 312040(Human)
• Uniprot:
  • NP_058633(Human)