DNA-PKcs [p Thr2609] Antibody - BSA Free

Western Blot: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Detection of a 460 kD band corresponding to human DNA-PKcs in various preparations. Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNA-PKcs inhibitor + IR, Lane 4: MO59J ( DNA-PKcs-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNA-PKcs inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene.

Immunohistochemistry-Paraffin: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Staining of human prostate tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

DNA-PKcs [p Thr2609] Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for several weeks
• Immunogen: Synthetic peptide corresponding to aa surrounding Thr 2609 of human DNA-PKcs.
• Modification: p Thr2609
• Specificity: This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA-PKcs. Reactivity with non-phosphorylated human DNA-PKcs is minimal. However, cross-reactivity is expected with rat, canine, chicken and equine DNA-PKcs based on a high degree of homology to the immunogen sequence.
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKDC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:300000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Application Notes: IHC: NBP1-02456 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Use in Flow Cytometry was reported in a scientific publication (PMID: 23691119).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23691).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 0.93 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA-PKcs [p Thr2609] Antibody - BSA Free

• DNA-dependent protein kinase catalytic subunit-like
• DNA-dependent protein kinase
• DNA-PK catalytic subunit
• DNAPK
• DNAPKcs
• DNA-PKcs
• DNPK1
• EC 2.7.11.1
• HYRC
• HYRC1
• p350
• p460
• PRKDC
• XRCC7

Background

DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA-PKcs Antibody (NBP1-02456)(6)

Publications using NBP1-02456

• Li RY, Yang D, He YJ et al. Heavy metal ions exchange driven protein phosphorylation cascade functions in genomic instability in spermatocytes and male infertility Nucleic acids research 2023-03-03 [PMID: 36869674] (Western Blot, Mouse)
• Fang T, Zhang Y, Chang VY et al. Epidermal growth factor receptor-dependent DNA repair promotes murine and human hematopoietic regeneration Blood 2020-05-05 [PMID: 32369572]
• Schuler N, Rube CE. Accumulation of DNA Damage-Induced Chromatin Alterations in Tissue-Specific Stem Cells: The Driving Force of Aging? PLoS One 2013-05-17 [PMID: 23691119] (FLOW, Mouse)
• Lorat Y, Schanz S, Schuler N, Wennemuth G, Rube C et al. (2012) Beyond Repair Foci: DNA Double-Strand Break Repair in Euchromatic and Heterochromatic Compartments Analyzed by Transmission Electron Microscopy. PLoS ONE 7(5): e38165. 2012-05-31 [PMID: 22666473] (Mouse)
• Fang T The Role of Epidermal Growth Factor Receptor Signaling in Hematopoietic Stem Cell Regeneration Thesis 2020-01-01
• Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications 2022-01-14 [PMID: 35031618] (WB, Chemotaxis)

Bioinformatics

• Gene Symbol: PRKDC
• Entrez:
  • 5591(Human)
  • 19090(Mouse)
  • 360748(Rat)
• Uniprot:
  • P78527()