Western Blot: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Detection of a 460 kD band corresponding to human DNA-PKcs in various preparations. Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNA-PKcs ...read more
Immunohistochemistry-Paraffin: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Staining of human prostate tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Synthetic peptide corresponding to aa surrounding Thr 2609 of human DNA-PKcs.
This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal. However, cross-reactivity is expected with rat, canine, chicken and equine DNA PKcs based on a high degree of homology to the immunogen sequence.
Chimpanzee (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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IHC: NBP1-02456 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Use in Flow Cytometry was reported in a scientific publication (PMID: 23691119).
Read Publications using NBP1-02456 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 23691).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
0.01% Sodium Azide
Immunogen affinity purified
Alternate Names for DNA-PKcs [p Thr2609] Antibody
DNA-dependent protein kinase catalytic subunit-like
DNA-dependent protein kinase
DNA-PK catalytic subunit
DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.