DNA-PKcs [p Thr2609] Antibody

Images

 
Western Blot: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Detection of a 460 kD band corresponding to human DNA-PKcs in various preparations. Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNA-PKcs ...read more
Immunohistochemistry-Paraffin: DNA-PKcs [p Thr2609] Antibody [NBP1-02456] - Staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DNA-PKcs [p Thr2609] Antibody Summary

Immunogen
Synthetic peptide from human PRKDC / DNA-PKcs.
Modification
p Thr2609
Specificity
aa 2604-2614 of human DNA PKcs.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKDC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:2000
  • ELISA 1:1000-1:5000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Use in Flow Cytometry was reported in a scientific publication (PMID: 23691119).
Publications
Read Publications using
NBP1-02456 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23691).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA-PKcs [p Thr2609] Antibody

  • DNA-dependent protein kinase catalytic subunit-like
  • DNA-dependent protein kinase
  • DNA-PK catalytic subunit
  • DNAPK
  • DNAPKcs
  • DNA-PKcs
  • DNPK1
  • EC 2.7.11.1
  • HYRC
  • HYRC1
  • p350
  • p460
  • PRKDC
  • XRCC7

Background

DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA-PKcs Antibody (NBP1-02456)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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Bioinformatics

Gene Symbol PRKDC
Entrez
Uniprot