DNA-PKcs Antibody (3H6) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

DNA-PKcs Antibody (3H6) [Alexa Fluor™ Plus 594] Summary

• Immunogen: Purified recombinant fragment of human DNA PKcs expressed in E. coli. [Uniprot: P78527]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKDC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DNA-PKcs Antibody (3H6) [Alexa Fluor™ Plus 594]

• DNA-dependent protein kinase catalytic subunit-like
• DNA-dependent protein kinase
• DNA-PK catalytic subunit
• DNAPK
• DNAPKcs
• DNA-PKcs
• DNPK1
• EC 2.7.11.1
• HYRC
• HYRC1
• p350
• p460
• PRKDC
• XRCC7

Background

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKDC