DNA-PKcs Antibody (18-2)

Images

 
Western Blot: DNA-PKcs Antibody (18-2) [NB600-1203] - Analysis of LS174T Cells.
Immunohistochemistry-Paraffin: DNA-PKcs Antibody (18-2) [NB600-1203] - Human tonsil stained with DNA-PKcs using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes.
Immunoprecipitation: DNA-PKcs Antibody (18-2) [NB600-1203] - Analysis of denature IP.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP, IF
Clone
18-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

DNA-PKcs Antibody (18-2) Summary

Immunogen
Human DNA-dependent Protein Kinase purified from HeLa cells
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKDC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 100
  • Immunoprecipitation 2 ug/ml
  • Immunofluorescence 1:10 - 1:500
Publications
Read Publication using NB600-1203.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for DNA-PKcs Antibody (18-2)

  • DNA-dependent protein kinase catalytic subunit-like
  • DNA-dependent protein kinase
  • DNA-PK catalytic subunit
  • DNAPK
  • DNAPKcs
  • DNA-PKcs
  • DNPK1
  • EC 2.7.11.1
  • HYRC
  • HYRC1
  • p350
  • p460
  • PRKDC
  • XRCC7

Background

DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA-PKcs Antibody (NB600-1203)(1)

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Bioinformatics

Gene Symbol PRKDC
Entrez
Uniprot