DLL4 Antibody (207822) [Unconjugated]

DLL4 was detected in immersion fixed frozen sections of mouse embryonic heart using Rat Anti-Human/Mouse DLL4 Monoclonal Antibody (Catalog # MAB1389) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing cardiomyocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 207822
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

DLL4 Antibody (207822) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse DLL4
  Ser28-Pro525
  Accession # Q9JI71
• Specificity: Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 100% cross-reactivity with recombinant human DLL4.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: DLL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLL4 Antibody (207822) [Unconjugated]

• Delta 4 precursor
• delta 4
• delta ligand 4
• delta4
• delta-like 4 (Drosophila)
• delta-like 4 homolog (Drosophila)
• delta-like 4 homolog
• delta-like 4 protein
• delta-like protein 4
• DLL4
• Drosophila Delta homolog 4
• hdelta2
• MGC126344
• notch ligand delta-2
• notch ligand DLL4

Background

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
2. Iso, T. et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
3. Walker, L. et al. (2001) Stem Cells 19:543.
4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DLL4 Antibody (MAB1389)(10)

Publications using MAB1389

• Adam Leach, Peter Smyth, Laura Ferguson, John Steven, Michelle K. Greene, Cristina M. Branco et al. Anti-DLL4 VNAR targeted nanoparticles for targeting of both tumour and tumour associated vasculature Nanoscale 2020-07-21 [PMID: 32626858]
• ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling Cell Death Dis, 2020-05-04;11(5):307. 2020-05-04 [PMID: 32366833] (Western Blot, Human)
• Park MH, Kim AK, Manandhar S et al. CCN1 interlinks integrin and Hippo pathway to autoregulate tip cell activity Elife 8/20/2019 [PMID: 31429823]
• M Verma, Y Asakura, BSR Murakonda, T Pengo, C Latroche, B Chazaud, LK McLoon, A Asakura Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling Cell Stem Cell, 2018-10-04;23(4):530-543.e9. 2018-10-04 [PMID: 30290177]
• Sarah De Val MEF2 transcription factors are key regulators of sprouting angiogenesis Genes Dev., 2016-10-15;30(20):2297-2309. 2016-10-15 [PMID: 27898394] (IHC, Mouse)
• Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development Elife, 2016-08-09;5(0):. 2016-08-09 [PMID: 27504807] (IHC-Fr, Western Blot, Mouse)
• Walchli T, Mateos J, Weinman O, Babic D, Regli L, Hoerstrup S, Gerhardt H, Schwab M, Vogel J Quantitative assessment of angiogenesis, perfused blood vessels and endothelial tip cells in the postnatal mouse brain. Nat Protoc, 2014-12-11;10(1):53-74. 2014-12-11 [PMID: 25502884] (IHC, Mouse)
• Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G The alarmin IL-33 is a notch target in quiescent endothelial cells. Am J Pathol, 2012-07-16;181(3):1099-111. 2012-07-16 [PMID: 22809957] (ICC, Human)
• Liu R, Li X, Tulpule A, Zhou Y, Scehnet JS, Zhang S, Lee JS, Chaudhary PM, Jung J, Gill PS KSHV-induced notch components render endothelial and mural cell characteristics and cell survival. Blood, 2009-11-24;115(4):887-95. 2009-11-24 [PMID: 19965636] (Western Blot, Human)
• Skokos D, Nussenzweig MC CD8- DCs induce IL-12-independent Th1 differentiation through Delta 4 Notch-like ligand in response to bacterial LPS. J. Exp. Med., 2007-06-18;204(7):1525-31. 2007-06-18 [PMID: 17576775] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: DLL4
• Uniprot:
  • Q9JI71()