DLL1 Antibody [Unconjugated]

DLL1 was detected in immersion fixed frozen sections of embryonic mouse stomach (E13.5) using 10 µg/mL Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3970) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Mouse splenocytes were stained with Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3970, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

• Reactivity: Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

DLL1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat DLL1
  Gln18-Trp537 (predicted)
  Accession # P97677
• Specificity: Detects rat and mouse DLL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human DLL‑1 is observed.
• Details of Functionality: ActivityAssaywCitation not found None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DLL1

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 1 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLL1 Antibody [Unconjugated]

• delta (Drosophila)-like 1
• Delta 1
• Delta
• Delta1
• delta-like 1 (Drosophila)
• delta-like protein 1
• DL1
• DLL1
• Drosophila Delta homolog 1
• H-Delta-1

Background

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature rat DLL1 consists of a 520 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, rat DLL1 shares 90% and 95% aa sequence identity with human and mouse DLL1, respectively. It shares 26%, 36%, and 53% aa sequence identity with rat DLL2, 3, and 4, respectively. The ADAM9, 12, or 17- mediated proteolysis of DLL1 releases a 60 kDa ECD fragment and regulates the Notch-dependent proliferation of hematopoietic and myogenic progenitor cells (2-4). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent
gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (5). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (6). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (4, 7-13). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (14). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (15-16).

1. Bettenhausen, B. et al. (1995) Development 121:2407.
2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
3. Karanu, F.N. et al. (2001) Blood 97:1960.
4. Sun, D. et al. (2008) J. Cell Sci. 121:3815.
5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
6. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
7. Takahashi, Y. et al. (2003) Development 130:4259.
8. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
9. Kiernan, A.E. et al. (2005) Development 132:4353.
10. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
11. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
12. Shimojo, H. et al. (2008) Neuron 58:52.
13. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. USA 104:537.
14. Limbourg, A. et al. (2007) Circ. Res. 100:363.
15. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
16. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DLL1 Antibody (AF3970)(3)

Publications using AF3970

• F Tetzlaff, MG Adam, A Feldner, I Moll, A Menuchin, J Rodriguez-, D Sprinzak, A Fischer MPDZ promotes DLL4-induced Notch signaling during angiogenesis Elife, 2018;7(0):. 2018 [PMID: 29620522] (Western Blot, Mouse)
• Hatakeyama J, Wakamatsu Y, Nagafuchi A, Kageyama R, Shigemoto R, Shimamura K Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates. Development, 2014;141(8):1671-82. 2014 [PMID: 24715457] (IHC, Chicken)
• Kawaguchi A, Ikawa T, Kasukawa T, Ueda HR, Kurimoto K, Saitou M, Matsuzaki F Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis. Development, 2008;135(18):3113-24. 2008 [PMID: 18725516] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: DLL1
• Uniprot:
  • P97677()