DLL1 Antibodies: Novus Biologicals

AF5026

DLL1 Antibody

100 μg

AF5026

NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

DLL1 was detected in immersion fixed frozen sections of embryonic mouse kidney (E13.5) using 10 µg/mL Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5026) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # <a class=

NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="DLL1 was detected in immersion fixed frozen sections of embryonic mouse kidney (E13.5) using 10 µg/mL Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5026) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Dll1 and Dll4 are expressed in distinct patterns in the mice gastrointestinal epithelium.Immunohistochemistry of Dll1 and Dll4 were performed using mice small intestinal (A, B) and colonic tissues (D, E). Within the small intestinal epithelium, staining by anti-Dll1 antibody showed positive cells exclusively within the crypt (A, B), whereas staining by anti-Dll4 antibody showed positive cells both in the crypt and in the villi. Quantification of Dll1+ve and Dll4+ve intestinal epithelial cells (IECs) showed that Dll1+ve IECs are predominantly found within the crypt, whereas Dll4+ve IECs are found mostly in the villi (C). In the colonic epithelium, staining by anti-Dll1 antibody showed positive cells exclusively within the lower part of the crypt (D, E), whereas staining by anti-Dll4 antibody showed positive cells both in the lower- and upper part of the crypt, including the surface epithelium. Quantification of Dll1+ve and Dll4+ve IECs showed that Dll1+ve IECs are found exclusively within the lower half of the crypt, whereas Dll4+ve IECs are found both in the lower and upper region of the crypt at a comparable frequency (F). Negative staining of non-immunized isotype antibodies (Control Ab) confirmed the specific staining of Dll1 (A) and Dll4 (D). Scale bar represents 20 µm. Quantitative data are shown as mean ∓ SD of triplicate experiments (n = 3). ∗ indicates P < 0.05 as determined by Welch’s t-test. N.S. indicates not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Dll1+ve or Dll4+ve IECs constitute the intestinal stem cell niche.Double immunostaining of Dll1 or Dll4 with a stem-cell niche cell marker, Lysozyme or c-kit, was performed in the mice small intestine and in the colon, respectively. (A) Immunostaining of Dll4 (green) with Lysozyme (red) shows that Dll4 is expressed in Paneth cells of the small intestine (yellow arrowhead). Scale bar represents 20 µm. (B) Double immunostaining of Dll1 (green) and c-kit (red) shows that Dll1+ve colonic IECs mostly co-express c-kit (yellow arrowhead). Also, double immunostaining of Dll4 (green) and c-kit (red) showed that a distinct population of Dll4+ve cells co-express c-kit (yellow arrowhead). Scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Dll4+ve cells dominate the colonic crypts of the DSS-colitis mice.Immunohistochemical analysis of colonic tissues that prepared from DSS-colitis mice is shown. (A) Immunostaining of Dll1&Dll4 using the inflamed colonic tissue of the DSS-colitis mice at day 10 (Colitis), or the corresponding tissue of the control mice (Control). In the inflamed colon, Dll1+ve IECs (green, upper panel) rarely found within the crypts (yellow arrowhead). In sharp contrast, Dll4+ve IECs frequently found, which dominated the entire crypt&also the surface epithelium. A magnified view of the area marked with a white square is shown in the right-end panel. Scale bar represents 50 µm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Both Dll1+ve and Dll4+ve IECs are mostly post-mitotic in the small intestine and in the colon.Double immunostaining of Dll1 or Dll4 (green) with the proliferation cell marker, Ki67 (red), shows that both Dll1+ve and Dll4+ve IECs are mostly post-mitotic in the small intestine (A) and in the colon (B). However, a small number of Dll1+ve or Dll4+ve IECs that co-express Ki67 can be found (yellow arrowhead). Scale bar represents 20 µm. These data were acquired by confocal microscopy (FV10i). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5026U-100UG
Alexa Fluor 405	AF5026V-100UG
Alexa Fluor 488	AF5026G-100UG
Alexa Fluor 532	AF5026X-100UG
Alexa Fluor 594	AF5026T-100UG
Alexa Fluor 647	AF5026R-100UG
Alexa Fluor 700	AF5026N-100UG
Alexa Fluor 750	AF5026S-100UG
Alexa Fluor Plus 405	AF5026AFP405-100UG
Alexa Fluor Plus 488	AF5026AFP488-100UG
Alexa Fluor Plus 555	AF5026AFP555-100UG
Alexa Fluor Plus 594	AF5026AFP594-100UG
Alexa Fluor Plus 647	AF5026AFP647-100UG
Alexa Fluor Plus 680	AF5026AFP680-100UG
Allophycocyanin	AF5026A
Biotin	AF5026B
CoraFluor 1	AF5026CL1
DyLight 350	AF5026D
DyLight 405	AF5026E
DyLight 488	AF5026K
DyLight 550	AF5026L
DyLight 594	AF5026M
DyLight 650	AF5026W
DyLight 680	AF5026Y
DyLight 755	AF5026Z
FITC	AF5026F
HRP	AF5026H
Janelia Fluor 525	AF5026JF525
Janelia Fluor 549	AF5026I
Janelia Fluor 646	AF5026J
Janelia Fluor 669	AF5026JF669
PE	AF5026P
PerCP	AF5026C
mFluor Violet 450 SE	AF5026MFV450
mFluor Violet 500 SE	AF5026MFV500
mFluor Violet 610 SE	AF5026MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5026-SP

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AF3970

DLL1 Antibody [Unconjugated]

(7 Publications)

100 μg

AF3970

5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (NL011)." class="big_lightbox">

5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (NL011)." alt="Mouse splenocytes were stained with Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3970, filled histogram) or control antibody (5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (NL011)." class="big_thumb" />

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

DLL1 was detected in immersion fixed frozen sections of embryonic mouse stomach (E13.5) using 10 µg/mL Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3970) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; <a class=

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Mpdz does not affect cell cell junction assembly.(A, B) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Cells were cultured under sparse conditions (A) or confluent conditions (B). After PFA fixation cells were stained for DLL1 and Nectin-2 or DLL4 and Nectin-2 and counterstained with DAPI. Images were acquired with the confocal microscope LSM 700. Arrow indicates co-localization of DLL1/4 with Nectin-2 at the cell membrane. Arrow head indicates diminished co-localization at the cell membrane. Scale bar: 10 µm. (C) HUVECs were either transfected with control siRNA (si-ctrl) or with siRNA against Nectin-2 (si-Nectin-2). After PFA fixation cells were stained for DLL1 and Nectin-2 or DLL4 and Nectin-2. Images were acquired with the confocal microscope LSM 700. Arrow indicates localization of DLL1/4 at the cell membrane.Scale bar: 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29620522), licensed under a CC-BY license. Not internally tested by R&D Systems.

MPDZ promotes Notch signaling activity.(A) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Expression level of Notch target genes HEY1, HEY2 and HES1 were analyzed by qPCR 48 hr after transduction. Data are presented as mean ±SD. n ≥ 3; *, p<0.05; **, p<0.01; ***, p<0.001 unpaired Student’s t-test. (B) Cardiac endothelial cells were isolated from Mpdzfl/fl and Mpdz delta EC mice by magnetic beads bound with CD31 antibodies. Expression levels of Notch target genes Hey1 and Hey2 were analyzed by qPCR. Data are presented as mean ±SD. n = 3; *, p<0.05; ***, p<0.001 unpaired Student’s t-test. (C) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Expression levels of DLL1 and DLL4 were analyzed by immunoblotting 48 hr after transduction. beta -actin served as loading control. Data are presented as mean ±SD. n ≥ 3; n.s., not significant. (D) HUVECs were either transduced with adenovirus expressing GFP (ctrl) or with adenovirus expressing MPDZ. Expression levels of DLL1 and DLL4 were analyzed by immunoblotting 48 hr after transduction. beta -actin served as loading control. Data are presented as mean ±SD. n ≥ 3; n.s., not significant. (E) Lung endothelial cells were isolated from Mpdzfl/fl and Mpdz delta EC mice by CD31 magnetic beads. Protein amounts of Dll1 and Dll4 were analyzed by immunoblotting. beta -actin served as loading control. Data are presented as mean ±SD. n = 3; n.s., not significant.10.7554/eLife.32860.007Figure 2—source data 1.Source data of qantitative PCR analysis related to Figure 2A and B.Source data of qantitative PCR analysis related to Figure 2A and B. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29620522), licensed under a CC-BY license. Not internally tested by R&D Systems.

5-001-A, open histogram) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

CHO cells transfected with DLL1 were stained with Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3970, filled histogram) or isotype control antibody (Catalog # <a class=

5-001-A, open histogram) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

HAF016) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

DLL1 was detected in perfusion fixed frozen sections of Embryonic Mouse Stomach (E13) using Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3970) at 1 µg/ml overnight at 4 °C. Tissue was stained using the HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Applications:

, , ,

Applications: , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3970U-100UG
Alexa Fluor 405	AF3970V-100UG
Alexa Fluor 488	AF3970G-100UG
Alexa Fluor 532	AF3970X-100UG
Alexa Fluor 594	AF3970T-100UG
Alexa Fluor 647	AF3970R-100UG
Alexa Fluor 700	AF3970N-100UG
Alexa Fluor 750	AF3970S-100UG
Alexa Fluor Plus 405	AF3970AFP405-100UG
Alexa Fluor Plus 488	AF3970AFP488-100UG
Alexa Fluor Plus 555	AF3970AFP555-100UG
Alexa Fluor Plus 594	AF3970AFP594-100UG
Alexa Fluor Plus 647	AF3970AFP647-100UG
Alexa Fluor Plus 680	AF3970AFP680-100UG
Allophycocyanin	AF3970A
Biotin	AF3970B
CoraFluor 1	AF3970CL1
DyLight 350	AF3970D
DyLight 405	AF3970E
DyLight 488	AF3970K
DyLight 550	AF3970L
DyLight 594	AF3970M
DyLight 650	AF3970W
DyLight 680	AF3970Y
DyLight 755	AF3970Z
FITC	AF3970F
HRP	AF3970H
Janelia Fluor 525	AF3970JF525
Janelia Fluor 549	AF3970I
Janelia Fluor 646	AF3970J
Janelia Fluor 669	AF3970JF669
PE	AF3970P
PerCP	AF3970C
mFluor Violet 450 SE	AF3970MFV450
mFluor Violet 500 SE	AF3970MFV500
mFluor Violet 610 SE	AF3970MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3970-SP

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MAB18181

DLL1 Antibody (251123) [Unconjugated]

(4 Publications)

100 μg

MAB18181

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

DLL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB18181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="DLL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB18181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

DLL1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB18181) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Expression pattern of DLL1 in the developing cochlea of primates. (a) In E87 cochlea, no expression of DLL1 was detected in either ATOH1-positive hair cells or SOX2-positive supporting cells. (b) In contrast, a relatively weak expression of DLL1 was observed in ATOH1-positive hair cells in the E92 cochlea. (c) In E96 cochlea, the expression of DLL1 in hair cells became more pronounced. (d–h) After E101, DLL1 was continuously expressed in hair cells. Nuclei were counterstained with Hoechst (blue). Scale bar: 20 μm. IHC, inner hair cell; OHC, the outer hair cell. (a,b,d,g,h): basal turn, (c,e,f): middle turn. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37351521), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB18181U-100UG
Alexa Fluor 405	FAB18181V-100UG
Alexa Fluor 488	FAB18181G-100UG
Alexa Fluor 532	FAB18181X-100UG
Alexa Fluor 594	FAB18181T-100UG
Alexa Fluor 647	FAB18181R-100UG
Alexa Fluor 700	FAB18181N-100UG
Alexa Fluor 750	FAB18181S-100UG
Alexa Fluor Plus 405	FAB18181AFP405-100UG
Alexa Fluor Plus 488	FAB18181AFP488-100UG
Alexa Fluor Plus 555	FAB18181AFP555-100UG
Alexa Fluor Plus 594	FAB18181AFP594-100UG
Alexa Fluor Plus 647	FAB18181AFP647-100UG
Alexa Fluor Plus 680	FAB18181AFP680-100UG
Allophycocyanin	FAB18181A
Biotin	FAB18181B
CoraFluor 1	FAB18181CL1
DyLight 350	FAB18181D
DyLight 405	FAB18181E
DyLight 488	FAB18181K
DyLight 550	FAB18181L
DyLight 594	FAB18181M
DyLight 650	FAB18181W
DyLight 680	FAB18181Y
DyLight 755	FAB18181Z
FITC	FAB18181F
HRP	FAB18181H
Janelia Fluor 525	FAB18181JF525
Janelia Fluor 549	FAB18181I
Janelia Fluor 646	FAB18181J
Janelia Fluor 669	FAB18181JF669
PE	FAB18181P
PerCP	FAB18181C
mFluor Violet 450 SE	FAB18181MFV450
mFluor Violet 500 SE	FAB18181MFV500
mFluor Violet 610 SE	FAB18181MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB18181-SP

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MAB1818

DLL1 Antibody (251127) [Unconjugated]

(3 Publications)

100 μg

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1818-SP
Carrier Free	MAB1818-500

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AF1818

DLL1 Antibody

(2 Publications)

100 μg

AF1818

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox">

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." alt="T98G human glioblastoma cell line was stained with Goat Anti-Human DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1818, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1818U-100UG
Alexa Fluor 405	AF1818V-100UG
Alexa Fluor 488	AF1818G-100UG
Alexa Fluor 532	AF1818X-100UG
Alexa Fluor 594	AF1818T-100UG
Alexa Fluor 647	AF1818R-100UG
Alexa Fluor 700	AF1818N-100UG
Alexa Fluor 750	AF1818S-100UG
Alexa Fluor Plus 405	AF1818AFP405-100UG
Alexa Fluor Plus 488	AF1818AFP488-100UG
Alexa Fluor Plus 555	AF1818AFP555-100UG
Alexa Fluor Plus 594	AF1818AFP594-100UG
Alexa Fluor Plus 647	AF1818AFP647-100UG
Alexa Fluor Plus 680	AF1818AFP680-100UG
Allophycocyanin	AF1818A
Biotin	BAF1818
CoraFluor 1	AF1818CL1
DyLight 350	AF1818D
DyLight 405	AF1818E
DyLight 488	AF1818K
DyLight 550	AF1818L
DyLight 594	AF1818M
DyLight 650	AF1818W
DyLight 680	AF1818Y
DyLight 755	AF1818Z
FITC	AF1818F
HRP	AF1818H
Janelia Fluor 525	AF1818JF525
Janelia Fluor 549	AF1818I
Janelia Fluor 646	AF1818J
Janelia Fluor 669	AF1818JF669
PE	AF1818P
PerCP	AF1818C
mFluor Violet 450 SE	AF1818MFV450
mFluor Violet 500 SE	AF1818MFV500
mFluor Violet 610 SE	AF1818MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1818-SP

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NB600-628

DLL1 Antibody - BSA Free

0.1 mg

NB600-628

Immunohistochemistry: DLL1 Antibody [NB600-628] - Immunohistochemistry of DLL1 antibody. Tissue: Mouse embryonic kidney.Fixation: formalin fixed paraffin embedded 10% conc.Antigen retrieval: pH 9.5. Primary antibody: 2ug/ml for 1.5 hour @ room Temp.Secondary antibody: Peroxidase secondary reagent for 45 min at RT.Localization: as shown.Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

ELISA: DLL1 Antibody [NB600-628] - ELISA results of purified DLL1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.

Immunohistochemistry of Rabbit Anti-Delta1 antibody. Tissue: Mouse embryonic kidney. Fixation: formalin fixed paraffin embedded 10% conc. Antigen retrieval: pH 9.5. Primary antibody: 2ug/ml for 1.5 hour @ room Temp. Secondary antibody: Peroxidase secondary reagent for 45 min at RT. Localization: as shown. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Immunohistochemistry of Rabbit anti-mouse DELTA1 Antibody in Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker (left, right), EDTA pH 9.5 Pressure Cooker (middle). Primary antibody: 2ug/ml 1.5 hour @ room temp. Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45

Reactivity:

Mu, Hu, Rt

Species Glossary

Reactivity: Mu, Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-59217

DLL1 Antibody - BSA Free

100 ul

NBP1-59217

Western Blot: DLL1 Antibody [NBP1-59217] - Hela cell lysate, Antibody Titration: 0.2-1 ug/ml

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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MAB3970

DLL1 Antibody (409005)

100 μg

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3970U-100UG
Alexa Fluor 405	FAB3970V-100UG
Alexa Fluor 488	FAB3970G-100UG
Alexa Fluor 532	FAB3970X-100UG
Alexa Fluor 594	FAB3970T-100UG
Alexa Fluor 647	FAB3970R-100UG
Alexa Fluor 700	FAB3970N-100UG
Alexa Fluor 750	FAB3970S-100UG
Alexa Fluor Plus 405	FAB3970AFP405-100UG
Alexa Fluor Plus 488	FAB3970AFP488-100UG
Alexa Fluor Plus 555	FAB3970AFP555-100UG
Alexa Fluor Plus 594	FAB3970AFP594-100UG
Alexa Fluor Plus 647	FAB3970AFP647-100UG
Alexa Fluor Plus 680	FAB3970AFP680-100UG
Allophycocyanin	FAB3970A
Biotin	FAB3970B
CoraFluor 1	FAB3970CL1
DyLight 350	FAB3970D
DyLight 405	FAB3970E
DyLight 488	FAB3970K
DyLight 550	FAB3970L
DyLight 594	FAB3970M
DyLight 650	FAB3970W
DyLight 680	FAB3970Y
DyLight 755	FAB3970Z
FITC	FAB3970F
HRP	FAB3970H
Janelia Fluor 525	FAB3970JF525
Janelia Fluor 549	FAB3970I
Janelia Fluor 646	FAB3970J
Janelia Fluor 669	FAB3970JF669
PE	FAB3970P
PerCP	FAB3970C
mFluor Violet 450 SE	FAB3970MFV450
mFluor Violet 500 SE	FAB3970MFV500
mFluor Violet 610 SE	FAB3970MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3970-SP

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NBP2-80048

DLL1 Antibody (D1L165-6) - Azide and BSA Free

100 μg

NBP2-80048

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-80048AF350
Alexa Fluor 405	NBP2-80048AF405
Alexa Fluor 488	NBP2-80048AF488
Alexa Fluor 532	NBP2-80048AF532
Alexa Fluor 594	NBP2-80048AF594
Alexa Fluor 647	NBP2-80048AF647
Alexa Fluor 700	NBP2-80048AF700
Alexa Fluor 750	NBP2-80048AF750
Allophycocyanin	NBP2-80048APC
Biotin	NBP2-80048B
CoraFluor 1	NBP2-80048CL1
DyLight 350	NBP2-80048UV
DyLight 405	NBP2-80048V
DyLight 488	NBP2-80048G
DyLight 550	NBP2-80048R
DyLight 594	NBP2-80048DL594
DyLight 650	NBP2-80048C
DyLight 680	NBP2-80048FR
DyLight 755	NBP2-80048IR
FITC	NBP2-80048F
HRP	NBP2-80048H
Janelia Fluor 525	NBP2-80048JF525
Janelia Fluor 549	NBP2-80048JF549
Janelia Fluor 646	NBP2-80048JF646
Janelia Fluor 669	NBP2-80048JF669
PE	NBP2-80048PE
PerCP	NBP2-80048PCP
mFluor Violet 450 SE	NBP2-80048MFV450
mFluor Violet 500 SE	NBP2-80048MFV500
mFluor Violet 610 SE	NBP2-80048MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80048-50ug

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NBP2-90620

DLL1 Antibody (019) - Azide and BSA Free

100 ul

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-90620AF350
Alexa Fluor 405	NBP2-90620AF405
Alexa Fluor 488	NBP2-90620AF488
Alexa Fluor 532	NBP2-90620AF532
Alexa Fluor 594	NBP2-90620AF594
Alexa Fluor 647	NBP2-90620AF647
Alexa Fluor 700	NBP2-90620AF700
Alexa Fluor 750	NBP2-90620AF750
Alexa Fluor Plus 405	NBP2-90620AFP405
Alexa Fluor Plus 488	NBP2-90620AFP488
Alexa Fluor Plus 555	NBP2-90620AFP555
Alexa Fluor Plus 594	NBP2-90620AFP594
Alexa Fluor Plus 647	NBP2-90620AFP647
Alexa Fluor Plus 680	NBP2-90620AFP680
Allophycocyanin	NBP2-90620APC
Biotin	NBP2-90620B
CoraFluor 1	NBP2-90620CL1
DyLight 350	NBP2-90620UV
DyLight 405	NBP2-90620V
DyLight 488	NBP2-90620G
DyLight 550	NBP2-90620R
DyLight 594	NBP2-90620DL594
DyLight 650	NBP2-90620C
DyLight 680	NBP2-90620FR
DyLight 755	NBP2-90620IR
FITC	NBP2-90620F
HRP	NBP2-90620H
Janelia Fluor 525	NBP2-90620JF525
Janelia Fluor 549	NBP2-90620JF549
Janelia Fluor 646	NBP2-90620JF646
Janelia Fluor 669	NBP2-90620JF669
PE	NBP2-90620PE
PerCP	NBP2-90620PCP
mFluor Violet 450 SE	NBP2-90620MFV450
mFluor Violet 500 SE	NBP2-90620MFV500
mFluor Violet 610 SE	NBP2-90620MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-90620-50ul

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NBP2-89936

DLL1 Antibody (306) - Azide and BSA Free

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89936AF350
Alexa Fluor 405	NBP2-89936AF405
Alexa Fluor 488	NBP2-89936AF488
Alexa Fluor 532	NBP2-89936AF532
Alexa Fluor 594	NBP2-89936AF594
Alexa Fluor 647	NBP2-89936AF647
Alexa Fluor 700	NBP2-89936AF700
Alexa Fluor 750	NBP2-89936AF750
Alexa Fluor Plus 488	NBP2-89936AFP488
Alexa Fluor Plus 555	NBP2-89936AFP555
Alexa Fluor Plus 594	NBP2-89936AFP594
Alexa Fluor Plus 647	NBP2-89936AFP647
Alexa Fluor Plus 680	NBP2-89936AFP680
Allophycocyanin	NBP2-89936APC
Biotin	NBP2-89936B
CoraFluor 1	NBP2-89936CL1
DyLight 350	NBP2-89936UV
DyLight 405	NBP2-89936V
DyLight 488	NBP2-89936G
DyLight 550	NBP2-89936R
DyLight 594	NBP2-89936DL594
DyLight 650	NBP2-89936C
DyLight 680	NBP2-89936FR
DyLight 755	NBP2-89936IR
FITC	NBP2-89936F
HRP	NBP2-89936H
Janelia Fluor 525	NBP2-89936JF525
Janelia Fluor 549	NBP2-89936JF549
Janelia Fluor 646	NBP2-89936JF646
Janelia Fluor 669	NBP2-89936JF669
PE	NBP2-89936PE
PerCP	NBP2-89936PCP
mFluor Violet 450 SE	NBP2-89936MFV450
mFluor Violet 500 SE	NBP2-89936MFV500
mFluor Violet 610 SE	NBP2-89936MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89936-50ul

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AF5026AFP680

DLL1 Antibody [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Conjugate:

Alexa Fluor Plus 680

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

DLL1 Antibodies

DLL1 Antibody

DLL1 Antibody [Unconjugated]

DLL1 Antibody (251123) [Unconjugated]

DLL1 Antibody (251127) [Unconjugated]

DLL1 Antibody

DLL1 Antibody - BSA Free

DLL1 Antibody - BSA Free

DLL1 Antibody (409005)

DLL1 Antibody (D1L165-6) - Azide and BSA Free

DLL1 Antibody (019) - Azide and BSA Free

DLL1 Antibody (306) - Azide and BSA Free

DLL1 Antibody [Alexa Fluor™ Plus 680]