DLGAP4 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

DLGAP4 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the center of human SAPAP4. The immunogen is located within amino acids 610 - 660 of SAPAP4. Amino Acid Squence: APAPEAPEPPPKHA
• Specificity: At least three isoforms of SAPAP2 are known to exist. This SAPAP4 antibody will not cross-react with other SAPAP proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLGAP4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Peptide affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for DLGAP4 Antibody [CoraFluor™ 1]

• DAP4FLJ20588
• discs, large (Drosophila) homolog-associated protein 4
• disks large associated protein 4
• disks large-associated protein 4
• DLP4
• KIAA0964MGC131862
• PSD-95/SAP90-binding protein 4
• RP5-977B1.6
• SAP90/PSD-95-associated protein 4
• SAPAP-4
• SAPAP4DAP-4

Background

DLGAP4 is part of the SAPAP family which is expressed in neuronal cells. It may play a role in molecular organization of synapses. DLGAP4 is known to have interactions with PIK3R1, BIN1, SRC, NCK1 and GRB2. Disease research is currently being studied with relation to DLGAP4 and ischemia, cerebritis, lung cancer, neuronitis, and transient cerebral ischemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLGAP4