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![Various whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLG1 antibody [GT31] (NBP3-48794) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-48794_mouse-dlg1-mab-gt31-western-blot-1411202518335918.jpg)
![Western Blot: DLG1 Antibody [NBP1-20907] - (0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DLG1-Antibody-Western-Blot-NBP1-20907-img0002.jpg "Western Blot: DLG1 Antibody [NBP1-20907] - (0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: DLG1 Antibody [NBP1-26611] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-SAP97 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SAP97 was also immunoprecipitated by another rabbit anti-SAP97 antibody, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/DLG1-Antibody-Western-Blot-NBP1-26611-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DLG1 Antibody [NBP1-26611] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-SAP97 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SAP97 was also immunoprecipitated by another rabbit anti-SAP97 antibody, which recognizes a downstream epitope.")