Dkk-3 Antibody

Images

 
Western Blot: Dkk-3 Antibody [NB100-899] - Staining of Human Cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Dkk-3 Antibody Summary

Immunogen
Peptide with sequence C-AAAALLGGEEI corresponding to C-Terminus according to NP_037385.2, NP_056965.3, NP_001018067.1.
Epitope
C-AAAALLGGEEI
Specificity
NP_037385.2; NP_056965.3 and NP_001018067.1 are varients of the same protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DKK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/mL
Application Notes
Western blot: Approx 38kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 38.4 kDa according to NP_037385.2).
Control
293 Whole Cell Lysate
Dkk-3 Overexpression Lysate
Publications
Read Publication using NB100-899.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dkk-3 Antibody

  • dickkopf (Xenopus laevis) homolog 3
  • dickkopf 3
  • dickkopf homolog 3 (Xenopus laevis)
  • Dickkopf-3
  • dickkopf-related protein 3
  • Dkk3
  • Dkk-3
  • hDkk-3
  • regulated in glioma
  • REIC
  • REICdkk-3
  • RIG
  • RIG-like 5-6
  • RIG-like 7-1

Background

DKK3 is a secreted protein that is a member of the dickkopf family. It contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-899
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Applications: WB, ELISA

Publications for Dkk-3 Antibody (NB100-899)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DKK3
Entrez
Uniprot