Dkk-3 Antibody

Western Blot: Dkk-3 Antibody [NB100-899] - Staining of Human Cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Dkk-3 Antibody Summary

• Immunogen: Peptide with sequence C-AAAALLGGEEI corresponding to C-Terminus according to NP_037385.2, NP_056965.3, NP_001018067.1.
• Epitope: C-AAAALLGGEEI
• Specificity: NP_037385.2; NP_056965.3 and NP_001018067.1 are varients of the same protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DKK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/mL
• Application Notes: Western blot: Approx 38kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 38.4 kDa according to NP_037385.2).
• Control:

  293 Whole Cell Lysate
  Dkk-3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Dkk-3 Antibody

• dickkopf (Xenopus laevis) homolog 3
• dickkopf 3
• dickkopf homolog 3 (Xenopus laevis)
• Dickkopf-3
• dickkopf-related protein 3
• Dkk3
• Dkk-3
• hDkk-3
• regulated in glioma
• REIC
• REICdkk-3
• RIG
• RIG-like 5-6
• RIG-like 7-1

Background

DKK3 is a secreted protein that is a member of the dickkopf family. It contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dkk-3 Antibody (NB100-899)(1)

Publication using NB100-899

• Krupnik VE, Sharp JD, Jiang C et al. Functional and structural diversity of the human Dickkopf gene family. Gene 1999-10-01 [PMID: 10570958]

Bioinformatics

• Gene Symbol: DKK3
• Entrez:
  • 27122(Human)
• Uniprot:
  • NP_037385.2(Human)