Dkk-2 Antibody

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Western Blot: Dkk-2 Antibody [NB300-810] - NB300-810 (2 ug/ml) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Dkk-2 Antibody Summary

Immunogen
Peptide with sequence ATYSSKARLHVCQKI corresponding to C-Terminus according to NP_055236.1.
Specificity
From sequence analysis it is possible that this product will crossreact with DKK4, unfortunately this has not yet been specifically tested.
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DKK2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 28 kDa band observed in human heart lysate (calculated MW of 28.5 kDa band according to NP_055236).
Publications
Read Publication using NB300-810.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dkk-2 Antibody

  • dickkopf (Xenopus laevis) homolog 2
  • dickkopf 2
  • dickkopf homolog 2 (Xenopus laevis)
  • dickkopf related protein-2
  • dickkopf-2
  • dickkopf-related protein 2
  • Dkk2
  • Dkk-2
  • hDkk-2

Background

Analysis of individual DKK domains and chimeric DKKs shows that the carboxy-terminal domains of both DKK1 and DKK2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. Xenopus Dickkopf 1 (DKK1) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of DKK have been identified in mammals. Each DKK molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of DKK1 and DKK2 inhibited Wnt3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of DKK2, but not that of DKK1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dkk-2 Antibody (NB300-810)(1)


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Bioinformatics

Gene Symbol DKK2
Entrez
Uniprot