After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
MAB003, open histogram) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
Species: Hu Applications: IHC, Flow, ICC/IF Host: Mouse Monoclonal
5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Human Dkk-2 (Catalog # 6628-DK) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a. The ED50 for this effect is 50-300 ng/mL (orange circles)." class="big_lightbox" target="_blank">
Analysis of individual DKK domains and chimeric DKKs shows that the carboxy-terminal domains of both DKK1 and DKK2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. Xenopus Dickkopf 1 (DKK1) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of DKK have been identified in mammals. Each DKK molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of DKK1 and DKK2 inhibited Wnt3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of DKK2, but not that of DKK1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.
![Immunocytochemistry Dkk-2 Antibody (994930) [Unconjugated]](http://images.novusbio.com/images/antibody/Dkk2_MAB6628_Immunocytochemistry__Immunofluorescence_23067.jpg)
![Bioactivity Dkk-2 [Unconjugated]](http://images.novusbio.com/images/protein/Dkk2_6628DK_1633.jpg)
![Immunohistochemistry Dkk-2 Antibody (994930) [Unconjugated]](http://images.novusbio.com/images/antibody/Dkk2_MAB6628_Immunohistochemistry_23068.jpg)
![Intracellular Staining by Flow Cytometry Dkk-2 Antibody (994930) [Unconjugated]](http://images.novusbio.com/images/antibody/Dkk2_MAB6628_Flow_Cytometry_22980.jpg)
