DISP1 Antibody

DISP1 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human DISP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3549) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

DISP1 Antibody Summary

• Immunogen: E. coli-derived recombinant human DISP1
  Glu212-Lys492, Ser746-Thr984
  Accession # NP_116279
• Specificity: Detects human DISP1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DISP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DISP1 Antibody

• DISP1
• DISPA
• DISPAFLJ43740
• dispatched A
• dispatched homolog 1 (Drosophila)
• Dispatched
• DKFZp434I0428
• MGC104180
• MGC13130
• MGC16796
• protein dispatched homolog 1

Background

DISP1 is a multispan (12) transmembrane protein with cytoplasmic N- and C-terminal tails. It contains a stero/sensing domain (SSD) and has been shown to play a role in regulating the release of cholesterol-modified SHH. Within the regions used for immunization, human and mouse DISP1 share 93% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DISP1 Antibody (AF3549)(1)

Publication using AF3549

• Kristina Ehring, Dominique Manikowski, Jonas Goretzko, Jurij Froese, Fabian Gude, Petra Jakobs et al. Conserved cholesterol-related activities of Dispatched 1 drive Sonic hedgehog shedding from the cell membrane Journal of Cell Science 2022-03-01 [PMID: 34308968]

Bioinformatics

• Gene Symbol: DISP1
• Uniprot:
  • NP_116279()