Dishevelled-1 Overexpression Lysate (Native)

Images

 
Western Blot: Dishevelled/Dvl1 Overexpression Lysate (Adult Normal) [NBL1-10061] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dishevelled/Dvl1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PAGE

Order Details

Dishevelled-1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC217691 and based on accession number NM_004421.
Epitope
C-terminal DDK tag
Specificity
Homo sapiens dishevelled, dsh homolog 1 (Drosophila) (DVL1), mRNA.
Gene
DVL1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
72.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Dishevelled-1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Dishevelled-1 Overexpression Lysate (Native)

  • dishevelled 1 (homologous to Drosophila dsh)
  • dishevelled, dsh homolog 1 (Drosophila)
  • Dishevelled1
  • Dishevelled-1
  • DSH homolog 1
  • Dsh
  • DVL
  • DVL1
  • DVL1L1
  • MGC54245
  • segment polarity protein dishevelled homolog DVL-1

Background

DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Dishevelled/Dvl1 Lysate (NBL1-10061) (0)

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Bioinformatics

Gene Symbol DVL1
Entrez