DIAPH1 Antibodies
We offer DIAPH1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each DIAPH1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DIAPH1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the DIAPH1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DIAPH1 polyclonal antibodies and browse our DIAPH1 monoclonal antibody catalog.
Alternate Names for DIAPH1 Antibodies
anti-DIAPH1 antibody, anti-DIA1 antibody, anti-diaphanous homolog 1 (Drosophila) antibody, anti-hDIA1 antibody, anti-homolog) 1 antibody, anti-LFHL1FLJ25265 antibody
9 Results for "diaph1" in Primary Antibodies
![Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.66 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NB600-231-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.66 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: DIAPH1 Antibody [NB600-231] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Diaphanous used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-NB600-231-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DIAPH1 Antibody [NB600-231] - Whole cell lysate (50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NB600-231-img0008.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NB600-231] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-Diaphanous1 used at a dilution of 1:100.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NB600-231-img0006.jpg)
![Western Blot: DIAPH1 Antibody [NB600-230] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Diaphanous 1 was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NB600-230-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DIAPH1 Antibody [NB600-230] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Diaphanous 1 was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: DIAPH1 Antibody [NB600-230] - Whole cell lysate (10 or 50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 and 0.04 ug/ml.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NB600-230-img0002.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0008.jpg "Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0005.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0006.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0007.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human spleen shows moderate cytoplasmic positivity in sinusoids.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0009.jpg)
![Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410] - Western blot analysis of extracts of 293T cells, using DIAPH1 Rabbit mAb (NBP3-15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-6N10D0-Western-Blot-NBP3-15410-img0001.jpg "Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410] - Western blot analysis of extracts of 293T cells, using DIAPH1 Rabbit mAb (NBP3-15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410] - Western blot analysis of extracts of various cell lines, using DIAPH1 Rabbit mAb (NBP3-15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-6N10D0-Western-Blot-NBP3-15410-img0002.jpg)
![Western Blot: DIAPH1 Antibody [NBP1-47202] - Analysis of NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-47202 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NBP1-47202-img0002.jpg "Western Blot: DIAPH1 Antibody [NBP1-47202] - Analysis of NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-47202 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: DIAPH1 Antibody - Azide and BSA Free [DIAPH1] - Western blot analysis of lysates from wild type (WT) and DIAPH1 knockdown (KD) 293T cells, using [KD Validated] DIAPH1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-03284_rabbit-polyclonal-diaph1-antibody-5220251636365.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DIAPH1 Antibody - Azide and BSA Free [DIAPH1] - Western blot analysis of lysates from wild type (WT) and DIAPH1 knockdown (KD) 293T cells, using [KD Validated] DIAPH1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Western Blot: DIAPH1 Antibody (1A8) [H00001729-M02] - Detection against Immunogen (37.18 KDa) .](http://images.novusbio.com/fullsize/DIAPH1-Antibody-1A8-Western-Blot-H00001729-M02-img0001.jpg "Western Blot: DIAPH1 Antibody (1A8) [H00001729-M02] - Detection against Immunogen (37.18 KDa) .")
![Sandwich ELISA: DIAPH1 Antibody (5A8) [H00001729-M01] - Detection limit for recombinant GST tagged DIAPH1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-5A8-Sandwich-ELISA-H00001729-M01-img0002.jpg "Sandwich ELISA: DIAPH1 Antibody (5A8) [H00001729-M01] - Detection limit for recombinant GST tagged DIAPH1 is approximately 0.1ng/ml as a capture antibody.")