DIAPH1 Antibody

Images

 
Western Blot: DIAPH1 Antibody [NB600-230] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB ...read more
Western Blot: DIAPH1 Antibody [NB600-230] - Whole cell lysate (10 or 50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 and 0.04 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

   

Genetic Strategies

   

DIAPH1 Antibody Summary

Immunogen
A synthetic peptide, which represented a portion of human diaphanous homolog 1 encoded within exon 1 (LocusLink ID 1729).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIAPH1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:30000
Application Notes
By Western Blot, this antibody recognizes a band at ~205 kDa, representing Diaphanous 1. *The investigator should determine the optimal working dilution for a specific application.
Publications
Read Publications using NB600-230.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DIAPH1 Antibody

  • DIA1
  • diaphanous homolog 1 (Drosophila)
  • hDIA1
  • homolog) 1
  • LFHL1FLJ25265

Background

Diaphanous 1 is a member of the Formin homology (FH) family of cytoskeletal organizer proteins. It is required for cytokinesis and transcriptional activation of the serum response factor. It has also been suggested to contribute to localized actin polymerization and stress fiber formation, both by recruiting profiling to sites of Rho action and by cooperating with ROCK. The human Diaphanous 1 gene is associated with Premature Ovarian Failure (POF) and Nonsyndromic Deafness (DFNA1), an autosomal dominant, fully penetrant progressive hearing loss.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DIAPH1 Antibody (NB600-230)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for DIAPH1 Antibody (NB600-230)

Discover related pathways, diseases and genes to DIAPH1 Antibody (NB600-230). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Calculators

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Bioinformatics

Gene Symbol DIAPH1
Entrez