DIAPH1 Antibody

Western Blot: DIAPH1 Antibody [NB600-230] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Diaphanous 1 was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).

Western Blot: DIAPH1 Antibody [NB600-230] - Whole cell lysate (10 or 50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 and 0.04 ug/ml.

• Reactivity: Hu
• Applications: WB, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

DIAPH1 Antibody Summary

• Immunogen: Maps to a region between residues 1 and 50 of human Diaphanous Homolog 1 using the numbering given in entry NP_005210.1 (GeneID 1729).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DIAPH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:10000-1:30000
• Application Notes: By Western Blot, this antibody recognizes a band at ~205 kDa, representing Diaphanous 1. *The investigator should determine the optimal working dilution for a specific application.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DIAPH1 Antibody

• DIA1
• diaphanous homolog 1 (Drosophila)
• hDIA1
• homolog) 1
• LFHL1FLJ25265

Background

Diaphanous 1 is a member of the Formin homology (FH) family of cytoskeletal organizer proteins. It is required for cytokinesis and transcriptional activation of the serum response factor. It has also been suggested to contribute to localized actin polymerization and stress fiber formation, both by recruiting profiling to sites of Rho action and by cooperating with ROCK. The human Diaphanous 1 gene is associated with Premature Ovarian Failure (POF) and Nonsyndromic Deafness (DFNA1), an autosomal dominant, fully penetrant progressive hearing loss.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DIAPH1 Antibody (NB600-230)(4)

Publications using NB600-230

• Colon-Franco JM, Gomez TS, Billadeau DD et al. Dynamic remodeling of the actin cytoskeleton by FMNL1gammais required for structural maintenance of the Golgi complex. J Cell Sci 2011-09-01 [PMID: 21868368]
• Ryu JR, Echarri A, Li R et al. Regulation of cell-cell adhesion by Abi/Diaphanous complexes. Mol Cell Biol 2009-04-01 [PMID: 19158278]
• Sakata D, Taniguchi H, Yasuda S et al. Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1. J Exp Med. 2007-09-03 [PMID: 17682067]
  
  Details:
  WB (Fig 5B): primary T cells isolated from adult mouse spleen.
• Minin AA, Kulik AV, Gyoeva FK et al. Regulation of mitochondria distribution by RhoA and formins. J Cell Sci 2006-02-01 [PMID: 16434478]

Bioinformatics

• Gene Symbol: DIAPH1
• Entrez:
  • 1729(Human)