DHCR7 Antibody

Western Blot: DHCR7 Antibody [NBP3-04053] - Analysis of extracts of various cell lines, using DHCR7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Product Discontinued

DHCR7 Antibody Summary

• Immunogen: A synthetic peptide of human DHCR7
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DHCR7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DHCR7 Antibody

• 7-dehydrocholesterol reductase
• 7-DHC reductase
• D7SR
• delta-7-dehydrocholesterol reductase
• EC 1.3.1
• EC 1.3.1.21
• Putative sterol reductase SR-2
• SLOS
• Smith-Lemli-Opitz syndrome
• Sterol Delta(7)-reductase
• sterol delta-7-reductase

Background

The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHCR7