DGK-iota Antibody [Janelia Fluor® 585]

Product Discontinued

DGK-iota Antibody [Janelia Fluor® 585] Summary

• Immunogen: Produced in rabbits immunized with E. coli-derived Human DGK-iota fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DGKI
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen and protein A Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for DGK-iota Antibody [Janelia Fluor® 585]

• DAG kinase iota
• DGKI
• DGKiota
• DGK-iota
• diacylglycerol kinase iota
• diacylglycerol kinase, iota
• Diglyceride kinase iota
• EC 2.7.1.107

Background

Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-alpha (80 kDa), -beta (90 kDa), and - gamma (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-delta (130 kDa) and DGK-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKI