DGCR8 [p Ser377] Antibody - Azide Free

Western Blot: DGCR8 [p Ser377] Antibody [NBP2-29530] - Western blot of mouse nuclei lysate showing specific immunolabeling of the ~120k DGCR8 protein phosphorylated at Ser377 (control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

DGCR8 [p Ser377] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit DGCR8 [p Ser377] Antibody - Azide Free (NBP2-29530) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 conjugated to KLH. Accession # Q8WYQ5
• Modification: p Ser377
• Specificity: Specific for endogenous levels of the ~120 kDa DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DGCR8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for DGCR8 [p Ser377] Antibody - Azide Free

• C22orf12
• DGCRK6chromosome 22 open reading frame 12
• DiGeorge syndrome critical region 8
• DiGeorge syndrome critical region gene 8
• Gy1
• microprocessor complex subunit DGCR8
• pasha

Background

The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGCR8
• Entrez:
  • 1644(Human)
• Uniprot:
  • Q8WYQ5(Human)