DGCR8 Antibody

Western Blot: DGCR8 Antibody [NBP1-30115] - Whole cell lysate from HeLa and 293T cells. DGCR8 was also IPed by rabbit anti-DGCR8 antibody NBP1-30116. For blotting immunoprecipitated DGCR8, the ReliaBLOT Reagents and Procedures were used.

Immunohistochemistry-Paraffin: DGCR8 Antibody [NBP1-30115] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DGCR8 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DGCR8 Antibody Summary

• Immunogen: Synthetic peptide, which maps to a region between residue 1 and 50 of human DiGeorge syndrome critical region gene 8 using the numbering given in entry NP_073557.3
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DGCR8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DGCR8 Antibody

• C22orf12
• DGCRK6chromosome 22 open reading frame 12
• DiGeorge syndrome critical region 8
• DiGeorge syndrome critical region gene 8
• Gy1
• microprocessor complex subunit DGCR8
• pasha

Background

DGCR8 is a component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-mi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DGCR8 Antibody (NBP1-30115)(1)

Publication using NBP1-30115

• Havens MA, Reich AA, Duelli DM, Hastings ML. Biogenesis of mammalian microRNAs by a non-canonical processing pathway. Nucleic Acids Research. 2012-01-23 [PMID: 22270084]

Bioinformatics

• Gene Symbol: DGCR8