DFF40/CAD Antibody (609809) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DFF40/CAD Monoclonal Antibody (Catalog # MAB6289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DFF40/CAD at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

DFF40/CAD was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DFF40/CAD Monoclonal Antibody (Catalog # MAB6289) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 609809
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DFF40/CAD Antibody (609809) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human DFF40/CAD
  Met1-Gln338
  Accession # O76075
• Specificity: Detects human DFF40/CAD in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DFFB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DFF40/CAD Antibody (609809) [Unconjugated]

• CAD
• CADCaspase-activated nuclease
• Caspase-activated deoxyribonuclease
• Caspase-activated DNase
• CPAN
• CPANDNA fragmentation factor 40 kDa subunit
• DFF2
• DFF40
• DFF-40DFF40DFF2DNA fragmentation factor subunit beta
• DFFB
• DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
• EC 3.-

Background

DNA fragmentatation factor 40 kDa subunit (DFF40), also known as CAD, is a member of the CIDE domain-containing proteins. It is an apoptotic nuclease that is maintained in an inactive state by association with DFF45. Apoptotic signaling induces DFF45 cleavage and the release of DFF40. Active DFF40 exists as an oligomer and generates double stranded breaks in dsDNA. Alternate splicing of human DFF40 generates isoforms with substitutions and truncations either directly following the CIDE domain (which is aa 4‑80) or at the C-terminus. Full length human DFF40 (aa 1‑338) shares 76% and 74% aa sequence identity with mouse and rat DFF40, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DFF40/CAD Antibody (MAB6289)(1)

Publication using MAB6289

• M Kijima, R Mizuta Histone H1 quantity determines the efficiencies of apoptotic DNA fragmentation and chromatin condensation Biomed. Res., 2019-01-01;40(1):51-56. 2019-01-01 [PMID: 30787263] (Western Blot, Human)

FAQs for DFF40/CAD Antibody (MAB6289). (Showing 1 - 1 of 1 FAQ).

1. Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?
   • CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

Bioinformatics

• Gene Symbol: DFFB
• Uniprot:
  • O76075(Human)