DFF40/CAD Antibody (609809) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 609809
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

DFF40/CAD Antibody (609809) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human DFF40/CAD
  Met1-Gln338
  Accession # O76075
• Specificity: Detects human DFF40/CAD in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DFF40/CAD Antibody (609809) [Alexa Fluor® 750]

• CAD
• CADCaspase-activated nuclease
• Caspase-activated deoxyribonuclease
• Caspase-activated DNase
• CPAN
• CPANDNA fragmentation factor 40 kDa subunit
• DFF2
• DFF40
• DFF-40DFF40DFF2DNA fragmentation factor subunit beta
• DFFB
• DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
• EC 3.-

Background

DNA fragmentatation factor 40 kDa subunit (DFF40), also known as CAD, is a member of the CIDE domain-containing proteins. It is an apoptotic nuclease that is maintained in an inactive state by association with DFF45. Apoptotic signaling induces DFF45 cleavage and the release of DFF40. Active DFF40 exists as an oligomer and generates double stranded breaks in dsDNA. Alternate splicing of human DFF40 generates isoforms with substitutions and truncations either directly following the CIDE domain (which is aa 4‑80) or at the C-terminus. Full length human DFF40 (aa 1‑338) shares 76% and 74% aa sequence identity with mouse and rat DFF40, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for DFF40/CAD Antibody (FAB6289S). (Showing 1 - 1 of 1 FAQ).

1. Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?
   • CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.