Desmoglein-1 Antibody (JB19-46)

Immunohistochemistry-Paraffin: Desmoglein-1 Antibody (JB19-46) [NBP2-75468] - Analysis of Desmoglein-1 antibody (JB19-46) on FFPE mouse skin tissue. Sections were retrieved in a pressure cooker with Tris-EDTA buffer. The antibody was applied for 30 min at RT at a concentration of .5 ug/ml. The antibody was detected with an anti-rabbit polymer and visualized with DAB. Image from verified customer review.

Immunohistochemistry-Paraffin: Desmoglein-1 Antibody (JB19-46) [NBP2-75468] - Analysis of paraffin-embedded human colon cancer tissue using anti-DSG1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Desmoglein-1 Antibody (JB19-46) [NBP2-75468] - Analysis of paraffin-embedded human skin tissue using anti-DSG1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Desmoglein-1 Antibody (JB19-46) [NBP2-75468] - Analysis of paraffin-embedded human uterus tissue using anti-DSG1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Desmoglein-1 Antibody (JB19-46) [NBP2-75468] - Analysis of paraffin-embedded mouse skin tissue using anti-DSG1 antibody. Counter stained with hematoxylin.

Product Discontinued

Desmoglein-1 Antibody (JB19-46) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Desmoglein-1 aa 1,000-1,049 / 1,049. (SwissProt: Q02413 Human; SwissProt: Q61495 Mouse)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DSG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Desmoglein-1 Antibody (JB19-46)

• Cadherin family member 4
• CDHF4
• CDHF4PPKS1
• desmoglein 1
• Desmoglein1
• Desmoglein-1
• Desmosomal glycoprotein 1
• DG1
• DGI
• DSG
• DSG1
• Pemphigus foliaceus antigen
• SPPK1

Background

Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. Desmoglein 1 has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Desmoglein-1 Antibody (NBP2-75468) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Michele Wich IHC-P Mouse 04/26/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: esophagus,skin
• Species: Mouse
• Lot: HL0222

Comments

• Comments: Sections were retrieved in a pressure cooker with Tris-EDTA buffer. The antibody was applied for 30 min at RT at a concentration of .5 ug/ml. The antibody was detected with an anti-rabbit polymer and visualized with DAB.
  I would have given it 5 stars, but the antibody is very unstable. Working dilution must be made up and used within a couple hours of preparation.

Bioinformatics

• Gene Symbol: DSG1