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Desmoglein-1 Antibody (DSG1/1733)

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Immunohistochemistry-Paraffin: Desmoglein-1 Antibody (DSG1/1733) [NBP2-53234] - Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-1 Monoclonal Antibody (DSG1/1733).
Immunohistochemistry-Paraffin: Desmoglein-1 Antibody (DSG1/1733) [NBP2-53234] - Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-1 Monoclonal Antibody (DSG1/1733).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clone
DSG1/1733
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54377AF350
Alexa Fluor 405
NBP2-54377AF405
Alexa Fluor 488
NBP2-54377AF488
Alexa Fluor 532
NBP2-54377AF532
Alexa Fluor 594
NBP2-54377AF594
Alexa Fluor 647
NBP2-54377AF647
Alexa Fluor 700
NBP2-54377AF700
Alexa Fluor 750
NBP2-54377AF750
Allophycocyanin
NBP2-54377APC
Allophycocyanin/Cy7
NBP2-54377APCCY7
Biotin
NBP2-54377B
DyLight 350
NBP2-54377UV
DyLight 405
NBP2-54377V
DyLight 488
NBP2-54377G
DyLight 550
NBP2-54377R
DyLight 594
NBP2-54377DL594
DyLight 650
NBP2-54377C
DyLight 680
NBP2-54377FR
DyLight 755
NBP2-54377IR
FITC
NBP2-54377F
HRP
NBP2-54377H
Janelia Fluor 549
NBP2-54377JF549
Janelia Fluor 646
NBP2-54377JF646
PE
NBP2-54377PE
PE/Atto594
NBP2-54377PEATT594
PE/Cy5.5
NBP2-54377PECY55
PE/Cy7
NBP2-54377PECY7
PerCP
NBP2-54377PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-54377-100ug
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Desmoglein-1 Antibody (DSG1/1733) Summary

Immunogen
Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain (exact sequence is proprietary)
Localization
Cell surface
Protein/Peptide Type
Recombinant Protein
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DSG1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes
IHC-P:Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Desmoglein-1 Antibody (DSG1/1733)

  • Cadherin family member 4
  • CDHF4
  • CDHF4PPKS1
  • desmoglein 1
  • Desmoglein1
  • Desmoglein-1
  • Desmosomal glycoprotein 1
  • DG1
  • DGI
  • DSG
  • DSG1
  • Pemphigus foliaceus antigen
  • SPPK1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Colocalisation of Dsg3 with E-cadherin-catenin complex. Co-IPs demonstrated that both endogenous and exogenous Dsg3 interacted with the E-cadherin-catenin complex in A431 stable lines (the empty vector control (V) and hDsg3.myc (D3)) as well as in HaCaTs. When pulled down with antibodies against the myc tag or with a mouse Dsg3 Ab 5H10 and blotted for E-cadherin and beta-catenin, both proteins were detected in D3 and, to a lesser extent, in V. Conversely, when pulled down with E-cadherin Ab HECD-1 and blotted for Dsg3 or the myc tag and Pg, these proteins were detected in both V and D3 cells. Note that this result also was confirmed in HaCaTs (HaC) where Dsg3 was expressed endogenously (far right blot). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0014211), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - The cultured primary mouse skin keratinocytes were stained at 1:150. The image shows the cytosolic staining. This image was submitted via customer review.
Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 on human skin cancer using NBP1-78984.
(1 Review)
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Western blot shows lysates of mouse skin tissue and mouse embryo (15 d.p.c.) tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Desmocollin‑3 Monoclonal Antibody (Catalog # MAB7265) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Desmocollin‑3 at approximately 95-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Desmocollin‑3 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Desmocollin‑3 Monoclonal Antibody (Catalog # MAB7265) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm in epidermis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Desmoglein-2
Antibody [Unconjugate
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Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Desmoglein‑2 at approximately 159-164 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Desmoglein‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF947) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Desmoglein‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF947) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Desmoglein‑2 at approximately 90-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Desmocollin-1
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Immunohistochemistry-Paraffin: Desmocollin-1 Antibody [NBP1-88099] - Staining of human skin shows moderate to strong membranous positivity in epidermal cells.
Immunohistochemistry-Paraffin: Desmocollin-1 Antibody [NBP1-88099] - Staining of human fallopian tube shows very weak membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Desmocollin-1 Antibody [NBP1-88099] - Staining of human prostate shows no membranous positivity in glandular cells.
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NBP1-85710
Immunohistochemistry-Paraffin: Desmocollin-2 Antibody [NBP1-85710] - Staining of human colon shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Desmocollin-2 Antibody [NBP1-85710] - Staining of human kidney shows low expression as expected.
Immunohistochemistry-Paraffin: Desmocollin-2 Antibody [NBP1-85710] - Staining in human esophagus and cerebral cortex tissues.. Corresponding DSC2 RNA-seq data are presented for the same tissues.
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Applications: IHC, IHC-P
E-Cadherin
Antibody [Unconjugate
AF748
E‑Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/MouseE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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RCAN1 Antibody
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Western Blot: RCAN1 Antibody [NBP1-46852] - Western blot analysis of human skeletal muscle tissue (A) using RCAN1 antibody at 2 ug/ml.
Immunohistochemistry: RCAN1 Antibody [NBP1-46852] - Immunohistochemical analysis of RCAN1 in human fetal brain.
Western Blot: RCAN1 Antibody [NBP1-46852] - Analysis of RCAN1 in human fetal brain.
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DSPP Antibody
NBP2-92546
Western Blot: DSPP Antibody [NBP2-92546] - Analysis of extracts of various cell lines, using DSPP at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Immunocytochemistry/Immunofluorescence: DSPP Antibody [NBP2-92546] - Analysis of U-2 OS cells using DSPP. Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: DSPP Antibody [NBP2-92546] - Analysis of H9C2 cells using DSPP. Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Corneodesmosin
Antibody
AF5725
Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Corneodesmosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5725) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Corneodesmosin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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NBP2-49306
Western Blot: Periplakin Antibody [NBP2-49306] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue
Immunohistochemistry-Paraffin: Periplakin Antibody [NBP2-49306] - Staining in human esophagus and skeletal muscle tissues using anti-PPL antibody. Corresponding PPL RNA-seq data are presented for the same tissues.
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Complement C3
Antibody (11H9)
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Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).
Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.
(2 Reviews)
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Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Cytokeratin 14
Antibody (LL002)
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Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Kallikrein 7
Antibody [Unconjugate
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Western blot shows lysates of human skin tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 7 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kallikrein 7 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human Kallikrein 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2624) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Dystonin Antibody
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Immunocytochemistry/Immunofluorescence: Dystonin Antibody [NBP1-89946] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Dystonin Antibody [NBP1-89946] - Staining of human tonsil shows low positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: Dystonin Antibody [NBP1-89946] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in Purkinje cells.
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Applications: ICC/IF, IHC, IHC-P
Collagen XVII
Antibody (SR46-05)
NBP2-67316
Western Blot: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Analysis of Collagen XVII on rat skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Staining Collagen XVII in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Collagen XVII Antibody (SR46-05) [NBP2-67316] - Human skin tissue using anti-Collagen XVII antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Kallikrein 5
[Unconjugated]
1108-SE
Recombinant Human Kallikrein 5 (Catalog # 1108-SE) is measured byits ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
(1 Review)
(17 Publications)
Species: Hu
gamma Catenin
Antibody (4C12)
NBP2-37557
Western Blot: gamma Catenin Antibody (4C12) [NBP2-37557] - Western blot analysis using JUP mouse mAb against T47D (1), MCF-7 (2), SKBR-3 (3), A431 (4) and HEK293 (5) cell lysate.
Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (4C12) [NBP2-37557] - Immunofluorescence analysis of U251 cells using JUP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: gamma Catenin Antibody (4C12) [NBP2-37557] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using JUP mouse mAb with DAB staining.
(2 Publications)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for Desmoglein-1 Antibody (NBP2-53234) (0)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Desmoglein-1 Antibody (NBP2-53234) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-54377AF350
Alexa Fluor 405 NBP2-54377AF405
Alexa Fluor 488 NBP2-54377AF488
Alexa Fluor 532 NBP2-54377AF532
Alexa Fluor 594 NBP2-54377AF594
Alexa Fluor 647 NBP2-54377AF647
Alexa Fluor 700 NBP2-54377AF700
Alexa Fluor 750 NBP2-54377AF750
Allophycocyanin NBP2-54377APC
Allophycocyanin/Cy7 NBP2-54377APCCY7
Biotin NBP2-54377B
DyLight 350 NBP2-54377UV
DyLight 405 NBP2-54377V
DyLight 488 NBP2-54377G
DyLight 550 NBP2-54377R
DyLight 594 NBP2-54377DL594
DyLight 650 NBP2-54377C
DyLight 680 NBP2-54377FR
DyLight 755 NBP2-54377IR
FITC NBP2-54377F
HRP NBP2-54377H
Janelia Fluor 549 NBP2-54377JF549
Janelia Fluor 646 NBP2-54377JF646
PE NBP2-54377PE
PE/Atto594 NBP2-54377PEATT594
PE/Cy5.5 NBP2-54377PECY55
PE/Cy7 NBP2-54377PECY7
PerCP NBP2-54377PCP
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Bioinformatics

Gene Symbol DSG1