Desmoglein-1 Antibody (32-2B)

Images

 
Immunohistochemistry-Paraffin: Desmoglein-1 Antibody (32-2B) [NBP3-07700] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Desmoglein-1 Mouse Monoclonal Antibody (32-2B).
Immunohistochemistry-Paraffin: Desmoglein-1 Antibody (32-2B) [NBP3-07700] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Desmoglein-1 Mouse Monoclonal Antibody (32-2B).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P
Clone
32-2B
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Desmoglein-1 Antibody (32-2B) Summary

Immunogen
Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis. (Uniprot: Q02413)
Localization
Cell surface
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DSG1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08662)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Desmoglein-1 Antibody (32-2B)

  • Cadherin family member 4
  • CDHF4
  • CDHF4PPKS1
  • desmoglein 1
  • Desmoglein1
  • Desmoglein-1
  • Desmosomal glycoprotein 1
  • DG1
  • DGI
  • DSG
  • DSG1
  • Pemphigus foliaceus antigen
  • SPPK1

Background

Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. Desmoglein 1 has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DSG1
Uniprot