Desert Hedgehog/Dhh Antibody - Azide and BSA Free

Western Blot: Desert Hedgehog/Dhh Antibody [H00050846-B01P] - Analysis of DHH expression in transfected 293T cell line by DHH polyclonal antibody. Lane 1: DHH transfected lysate(43.56 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Desert Hedgehog/Dhh Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Desert Hedgehog/Dhh Antibody - Azide and BSA Free (H00050846-B01P) is a polyclonal antibody validated for use in IHC and WB. Anti-Desert Hedgehog/Dhh Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: DHH (NP_066382.1, 1 a.a. - 396 a.a.) full-length human protein. MALLTNLLPLCCLALLALPAQSCGPGRGPVGRRRYARKQLVPLLYKQFVPGVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGGCFPGNATVRLWSGERKGLRELHRGDWVLAADASGRVVPTPVLLFLDRDLQRRASFVAVETEWPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSVLAPGGDALRPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESHQWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELLG
• Specificity: DHH - desert hedgehog homolog (Drosophila),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DHH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28881358).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Desert Hedgehog/Dhh Antibody - Azide and BSA Free

• desert hedgehog (Drosophila) homolog
• desert hedgehog homolog
• desert hedgehog protein
• Desert Hedgehog
• DHH
• HHG-3MGC35145

Background

This gene encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Desert Hedgehog/Dhh Antibody (H00050846-B01P)(3)

Publications using H00050846-B01P

• Joanna Pyczek, Natalia Khizanishvili, Maria Kuzyakova, Sebastian Zabel, Julia Bauer, Frauke Nitzki, Steffen Emmert, Michael P. Schön, Petra Boukamp, Hans-Ulrich Schildhaus, Anja Uhmann, Heidi Hahn Regulation and Role of GLI1 in Cutaneous Squamous Cell Carcinoma Pathogenesis Frontiers in Genetics 2019-12-04 [PMID: 31867038]
• Almazan-Moga A, Zarzosa P, Molist C et al. Ligand-dependent Hedgehog pathway activation in Rhabdomyosarcoma: the oncogenic role of the ligands. Br. J. Cancer. 2017-10-24 [PMID: 28881358] (IHC-P, Human)
• McKee CM, Xu D, Cao Y et al. Protease nexin 1 inhibits hedgehog signaling in prostate adenocarcinoma. J Clin Invest. 2012-10-08 [PMID: 23041623]

Bioinformatics

• Gene Symbol: DHH
• Entrez:
  • 50846(Human)
• Uniprot:
  • NP_066382.1(Human)