Recombinant Mouse Desert Hedgehog C23II Protein

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SDS-Page: Mouse Desert Hedgehog C23II Protein [NBP2-35175]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Mouse Desert Hedgehog C23II Protein Summary

Description
A biologically active protein to DHH.

Source: E. coli

Amino Acid Sequence: IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGSERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGVRLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGFDWVYYESRNH IHVSVKADNS LAVRAGG
Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
Desert Hedgehog Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3E1cells is 5-20 ug/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
DHH
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
20.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 1 mM DTT, 0.05 % Tween-80.
Concentration
LYOPH
Purity
> 98 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Background

Desert hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are cleaved autocatalytically, and the N-terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Dhh binds Patched, Patched 2, and Hedgehog interacting protein (Hip), inhibiting the ability of Patched to repress Smoothened signaling. Dhh is produced by Sertoli cells and is required for testis development, Leydig cell differentiation, and spermatogenesis. It is expressed in the female by ovarian granulosa cells and the corporus luteum and is upregulated in ovarian cancer. Dhh is also expressed by Schwann cells and is upregulated following nerve injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol DHH
Uniprot