Recombinant Mouse Desert Hedgehog C23II Protein

SDS-Page: Mouse Desert Hedgehog C23II Protein [NBP2-35175]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse Desert Hedgehog C23II Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 177 amino acids corresponding to Desert Hedgehog C23II Source: E. coli

  Uniprot ID: Q61488

  Amino Acid Sequence: IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG

• Details of Functionality: Desert Hedgehog C23II protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3E1 cells is 5-20 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DHH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Desert Hedgehog C23II as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 20.0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 1 mM DTT, 0.05 % Tween-80.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Background

Desert hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are cleaved autocatalytically, and the N-terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Dhh binds Patched, Patched 2, and Hedgehog interacting protein (Hip), inhibiting the ability of Patched to repress Smoothened signaling. Dhh is produced by Sertoli cells and is required for testis development, Leydig cell differentiation, and spermatogenesis. It is expressed in the female by ovarian granulosa cells and the corporus luteum and is upregulated in ovarian cancer. Dhh is also expressed by Schwann cells and is upregulated following nerve injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Desert Hedgehog C23II Protein (NBP2-35175)(1)

Publication using NBP2-35175

• Fulmer D, Toomer KA, Glover J et al. Desert Hedgehog-Primary Cilia Cross Talk Shapes Mitral Valve Tissue by Organizing Smooth Muscle Actin Dev. Biol. 2020-03-06 [PMID: 32151560]

Bioinformatics

• Gene Symbol: DHH
• Uniprot:
  • Q61488()
  • Q61488()
  • Q61488()